Optical coherence tomography (OCT) analysis was performed at pre-injury baseline and 5 weeks post-injury using a Spectralis spectral-domain OCT imaging system (Heidelberg Engineering, Heidelberg, Germany) and a 25-dipoter (D) lens for mouse ocular imaging (Heidelberg Engineering). Mice were anesthetized with a combination of ketamine (30 mg/kg, IP) and xylazine (5 mg/kg, IP) and placed on a heating pad to maintain body temperature. Pupils were dilated using a 1% tropicamide solution (Sandoz, Princeton, NJ, USA), and the cornea was moisturized with a balanced saline solution. After the recording, the cornea was moisturized with 0.3% hypromellose (GenTeal Tears, Alcon, Fort Worth, TX, USA). Volume scans (with a pattern size of 20° × 25° and 61 B-lines) were positioned directly over the optic nerve head to quantify the RGC complex thickness, which includes RGC bodies, axons, and dendrites. Scans in the superior retina were analyzed by a masked observer, excluding blood vessels from the RGC complex thickness calculation.
25 dipoter lens for mouse ocular imaging
Manufactured by Heidelberg Engineering
Sourced in Germany
The 25-dipoter (D) lens for mouse ocular imaging is a specialized optical component designed to facilitate the imaging of mouse eyes. It is a key part of the equipment used in research and clinical settings for the examination and analysis of the mouse visual system.
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Lab products found in correlation
2 protocols using 25 dipoter lens for mouse ocular imaging
1
Optical Coherence Tomography for Retinal Ganglion Cell Evaluation
2
Retinal Ganglion Cell Analysis in Mice
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The SD-OCT analysis was performed at pre-blast baseline and four weeks post-injury using a Spectralis SD-OCT (Heidelberg Engineering, Vista, CA) imaging system and a 25 dipoter (D) lens for mouse ocular imaging (Heidelberg Engineering). Mice were anesthetized with a combination of ketamine (0.03 mg/g, IP) and xylazine (0.005 mg/g, IP) and placed on a heating pad to maintain body temperature. Pupils were dilated using a 1% tropicamide solution, and the cornea was moisturized with saline. Volume scans (49 line dense array) positioned directly over the optic nerve head were performed to quantify the RGC complex thickness, which includes RGC bodies, axons, and dendrites.
Scans approximately 100 um from the edge of the optic nerve head were analyzed by a masked observer, excluding blood vessels from the RGC complex thickness calculation. Statistical significance was determined by one-way ANOVA with the Dunnett post-test for multiple comparisons.
Scans approximately 100 um from the edge of the optic nerve head were analyzed by a masked observer, excluding blood vessels from the RGC complex thickness calculation. Statistical significance was determined by one-way ANOVA with the Dunnett post-test for multiple comparisons.
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