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Poly acrylic acid sodium salt 5 100

Manufactured by Hampton Research

Poly (acrylic acid sodium salt) 5,100 is a water-soluble polymer that can be used as a viscosity modifier or dispersant in various applications. It has a molecular weight of approximately 5,100 g/mol.

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2 protocols using poly acrylic acid sodium salt 5 100

1

Structural Studies of EcPsuK with Pseudouridine and m1Ψ

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Apo-EcPsuK was crystallized using the hanging drop vapor diffusion method by mixing 1 μl of protein and 1 μL of reservoir solution at 18°C. The crystal suitable for X-ray diffraction was grown in a reservoir solution consisting of 0.02 M magnesium chloride hexahydrate, 0.1 M HEPES pH 7.5, 22% w/v poly (acrylic acid sodium salt) 5,100 (Hampton Research). For the EcPsuK-Ψ complex, the crystals suitable for data collection were first co-crystallized of EcPsuK with ADP and Ψ in the reservoir solution containing 20% (w/v) polyacrylic acid 5,100, 0.1 M HEPES/sodium hydroxide pH 7.0, 0.02 M magnesium chloride, and were further soaked with cryoprotectant containing the solution supplied with 25% glycerol, 1mM Ψ and 4mM ADP. For the EcPsuK-m1Ψ complex, the crystals suitable for data collection were first co-crystallized of EcPsuK with ADP and m1Ψ in the reservoir solution containing 20% (w/v) polyacrylic acid 5,100, 0.1M HEPES/sodium hydroxide pH 7.0, and 0.02M magnesium chloride and were further soaked with a cryoprotectant containing the solution supplied with 25% glycerol, 1mM m1Ψ, and 4mM ADP.
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2

Purification and Characterization of Membrane Proteins

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Arachidonic acid (AA; 5Z, 8Z, 11Z, 14Z-eicosatetraenoic acid) and aspirin were purchased from Cayman Chemical Company (Ann Arbor, MI). Celebrex® was from a physician sample. [acetyl-14C] aspirin (55 mCi/mmol) was purchased from American Radiolabeled Chemicals (St. Louis, MO). Fe3+-protoporphyrin IX and Co3+-protoporphyrin IX were purchased from Frontier Scientific (Logan, UT). Decyl maltoside (C10M) was purchased from Anatrace (Maumee, OH), while n-octyl-β-D-glucopyranoside (βOG) was purchased from Inalco Pharmaceuticals (San Luis Obispo, CA). Anti-FLAG M2 affinity resin was purchased from Sigma-Aldrich (St. Louis, MO). FLAG peptide was purchased from GenScript (Piscataway, NJ). Polyacrylic acid (sodium salt) 5100 was purchased from Hampton Research. Restriction enzymes were purchased form New England Biolabs. The QuikChange™ II mutagenesis kit was purchased from Agilent Technologies (Santa Clara, CA). Oligos for site-directed mutagenesis, the Bac-to-Bac® Baculovirus expression kit and associated reagents, including fungizone and penicillin-streptomycin, were purchased from Invitrogen (Carlsbad, CA). SFM 921 serum free media, Spodoptera frugiperda 21 (Sf21) insect cells, and fetal bovine serum were purchased from Expression Systems (Davis, CA). HiTrap HP chelating and HiPrep Sephacryl 300-HR chromatography columns were purchased from GE Healthcare.
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