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6210 time of flight lc ms system

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The Agilent 6210 time-of-flight LC/MS system is a high-performance mass spectrometer designed for accurate mass measurement and molecular formula determination. It features a time-of-flight mass analyzer that provides high resolution and mass accuracy for the identification and characterization of a wide range of compounds.

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Lab products found in correlation

400 mhz spectrometer, by Agilent Technologies (4 mentions) 6130 mass spectrometer, by Agilent Technologies (3 mentions) Masshunter software version b 02, by Agilent Technologies (3 mentions) Diode array detector, by Agilent Technologies (2 mentions) Luna c18 column, by Phenomenex (2 mentions) 1200 hplc, by Agilent Technologies (1 mentions) Agilent 1200 series, by Agilent Technologies (1 mentions) Zorbax eclipse xdb c18, by Agilent Technologies (1 mentions) Cesium carbonate, by Merck Group (1 mentions) Gemini phenyl column, by Phenomenex (1 mentions)

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LC-MS system (22 citations) 6210 Time of Flight LC/MS (2 citations) Agilent 6210 Time-of-Flight LC/MS system (2 citations)

5 protocols using 6210 time of flight lc ms system

1

Analytical Characterization of Chemical Compounds

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Compounds were synthesized in the National Center for Advancing Translational Sciences (Supporting Information Table S2). Purity determination was performed using an Agilent diode array detector for both method 1 and method 2 (below). Mass determination was performed using an Agilent 6130 mass spectrometer with electrospray ionization in the positive mode. Chiral separations were performed by normal phase chromatography using a CHIRALPAKAD column (5 cm × 50 cm, 20 μm) on an Agilent 1200 HPLC. 1H NMR spectra were recorded on Varian 400 MHz spectrometers. Chemical shifts are reported in ppm with undeuterated solvent (DMSO-d6 at 2.49 ppm) as internal standard for DMSO-d6 solutions. All of the analogs tested in the biological assays have purity greater than 95%, based on both analytical methods. High resolution mass spectrometry was recorded on Agilent 6210 time-of-flight LC/MS system. Confirmation of molecular formula was accomplished using electrospray ionization in the positive mode with the Agilent Masshunter software (version B.02).
Horton J.R., Liu X., Wu L., Zhang K., Shanks J., Zhang X., Rai G., Mott B.T., Jansen D.J., Kales S.C., Henderson M.J., Pohida K., Fang Y., Hu X., Jadhav A., Maloney D.J., Hall M.D., Simeonov A., Fu H., Vertino P.M., Yan Q, & Cheng X. (2018). Insights into the Action of Inhibitor Enantiomers against Histone Lysine Demethylase 5A. Journal of medicinal chemistry, 61(7), 3193-3208.
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2

HPLC-MS Analysis of Organic Analogs

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Analysis was performed on an Agilent 1260 with a 7 min gradient from 4% to 100% acetonitrile (containing 0.025% trifluoroacetic acid) in water (containing 0.05% trifluoroacetic acid) over 8 min run time at a flow rate of 1 mL/min. A Phenomenex Luna C18 column (3 µm, 3 mm × 75 mm) was used at a temperature of 50 °C. Purity determination was performed using an Agilent diode array detector for both method 1 and method 2. Mass determination was performed using an Agilent 6130 mass spectrometer with electrospray ionization in the positive mode. All of the analogs for assay have purity greater than 95% based on both analytical methods. 1H NMR spectra were recorded on Varian 400 MHz spectrometers. High resolution mass spectrometry results were recorded on Agilent 6210 time-of-flight LC/MS system.
Yang S.M., Yasgar A., Miller B., Lal-Nag M., Brimacombe K., Hu X., Sun H., Wang A., Xu X., Nguyen K., Oppermann U., Ferrer M., Vasiliou V., Simeonov A., Jadhav A, & Maloney D.J. (2015). Discovery of NCT-501, a Potent and Selective Theophylline-Based Inhibitor of Aldehyde Dehydrogenase 1A1 (ALDH1A1). Journal of medicinal chemistry, 58(15), 5967-5978.
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3

Mahonia aquifolium Stem Bark Extraction and Analysis

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The stem bark of cultivated Mahonia aquifolium (Pursh) Nutt. was collected in the National Garden park in Pančevo, Serbia, in October 2014. The voucher specimen is deposited at the herbarium of the Institute for Medicinal Plants Research “Dr Josif Pančić “, Belgrade (No. 046/14).
Both dry extracts were obtained from air-dried and finely powdered stem bark of cultivated M. aquifolium. MAE was extracted with 70% EtOH at room temperature for 24 h (1:5, w/v), while MAW was prepared by ultrasound-assisted extraction with water (1:10, w/v) for 30 min. Dry extracts were analyzed by the LC/MS method on Agilent 1200 Series, Agilent Technologies, with a DAD detector on the column Zorbax Eclipse XDB-C18 (RRHT, 50 × 4.6 mm i.d.; 1.8 μm) in combination with 6210 time-of-flight LC/MS system (Agilent Technologies) [14 (link)].
Damjanović A., Kolundžija B., Matić I.Z., Krivokuća A., Zdunić G., Šavikin K., Janković R., Stanković J.A, & Stanojković T.P. (2020). Mahonia aquifolium Extracts Promote Doxorubicin Effects against Lung Adenocarcinoma Cells In Vitro. Molecules, 25(22), 5233.
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4

Characterization of Small Molecules by LCMS

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All air or moisture-sensitive reactions were performed under a positive pressure of argon with oven-dried glassware. 4M HCl in dioxane and cesium carbonate were purchased from Sigma–Aldrich and used as such. Analytical analysis was performed on an Agilent LC/MS (Agilent Technologies). Method: A 7 min gradient of 4% to 100% acetonitrile (containing 0.025% trifluoroacetic acid [TFA]) in water (containing 0.05% TFA) was used with an 8 min run time at a flow rate of 1 ml/min. A Phenomenex Luna C18 column (3-micron, 3 × 75 mm) was used at a temperature of 50 °C. A Phenomenex Gemini Phenyl column (3-micron, 3 × 100 mm) was used at a temperature of 50 °C. Purity determination was performed using an Agilent Diode Array Detector for both Method 1 and Method 2. Mass determination was performed using an Agilent 6130 mass spectrometer with electrospray ionization in the positive mode. 1H NMR spectra were recorded on Varian 400 MHz spectrometers. Chemical shifts are reported in ppm with undeuterated solvent (DMSO-d6 at 2.49 ppm) as an internal standard for DMSO-d6 solutions. High-resolution mass spectrometry was recorded on Agilent 6210 Time-of-Flight LC/MS system. Confirmation of molecular formula was accomplished using electrospray ionization in the positive mode with the Agilent Masshunter software (version B.02).
Ciulla D.A., Dranchak P., Pezzullo J.L., Mancusi R.A., Psaras A.M., Rai G., Giner J.L., Inglese J, & Callahan B.P. (2022). A cell-based bioluminescence reporter assay of human Sonic Hedgehog protein autoprocessing to identify inhibitors and activators. The Journal of Biological Chemistry, 298(12), 102705.
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5

Analytical Characterization of Synthetic Compounds

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Compounds were synthesized at the National Center for Advancing Translational Sciences. Purity determination was performed using an Agilent Diode Array Detector for both Method 1 and Method 2 (below). Mass determination was performed using an Agilent 6130 mass spectrometer with electrospray ionization in the positive mode. 1H NMR spectra were recorded on Varian 400 MHz spectrometers. Chemical shifts for final compounds are reported in ppm with undeuterated solvent (DMSO-d6 at 2.50 ppm) as internal standard for DMSO-d6 solutions. Chemical shifts for intermediate compounds are reported in ppm with undeuterated solvent (CDCl3 at 7.26 ppm) as internal standard for CDCl3 solutions. All the analogs tested in the biological assays have purity greater than 95%, based on both analytical methods. High resolution mass spectrometry was recorded on Agilent 6210 Time-of-Flight LC/MS system. Confirmation of molecular formula was accomplished using electrospray ionization in the positive mode with the Agilent Masshunter software (version B.02).
Horton J.R., Woodcock C.B., Chen Q., Liu X., Zhang X., Shanks J., Rai G., Mott B.T., Jansen D.J., Kales S.C., Henderson M.J., Cyr M., Pohida K., Hu X., Shah P., Xu X., Jadhav A., Maloney D.J., Hall M.D., Simeonov A., Fu H., Vertino P.M, & Cheng X. (2018). Structure-Based Engineering of Irreversible Inhibitors against Histone Lysine Demethylase KDM5A. Journal of medicinal chemistry, 61(23), 10588-10601.
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