Pggt1b

Manufactured by Merck Group

Pggt1b is a laboratory equipment product. It serves a core function related to research and testing applications. Detailed information about its specific features and intended use is not available.

Automatically generated - may contain errors

Lab products found in correlation

Rap1a, by Santa Cruz Biotechnology (1 mentions) β actin, by Cell Signaling Technology (1 mentions) β tubulin 9f3, by Cell Signaling Technology (1 mentions) Gapdh, by Proteintech (1 mentions) β actin hrp, by Merck Group (1 mentions) Phosphor stat3 tyr705, by Cell Signaling Technology (1 mentions) Phospho akt s473, by Cell Signaling Technology (1 mentions) Phosphor erk, by Cell Signaling Technology (1 mentions) Phosphor smad2, by Cell Signaling Technology (1 mentions) Phosphor smad3, by Cell Signaling Technology (1 mentions)

2 protocols using pggt1b

Real-Time PCR and Immunoblot Analysis

Cited 1 time

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Real-time PCR analysis was performed as previously described with probe sets from Applied Biosystems (Du et al., 2018 (link)). Immunoblot analysis was performed as described previously (Du et al., 2018 (link)) using the following antibodies: p-Foxo1 (Thr24)/Foxo3a (Thr32; 9464), p-Mob1 (Thr35; D2F10), p-Pak1 (Thr423)/2(Thr402; 2601), β-tubulin (9F3), and β-actin (8H10D10; all from Cell Signaling Technology), Pggt1b (5E4; Sigma), Tiam1 (AF-5038, R&D Systems), Rap1a (SC-1482; Santa Cruz Biotechnology), Gapdh (1E6D9; Proteintech), and Hdj-2 (KA2A5.6; Lab Vision).

Du X., Zeng H., Liu S., Guy C., Dhungana Y., Neale G., Bergo M.O, & Chi H. (2019). Mevalonate metabolism–dependent protein geranylgeranylation regulates thymocyte egress. The Journal of Experimental Medicine, 217(2), e20190969.

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Comprehensive Protein Expression Analysis Protocol

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Cells were lysed with RIPA lysis buffer (25 mM Tris-HCl (pH 7.4), 150 mM NaCl, 5 mM EDTA, 0.1% SDS, 0.5% sodium deoxycholate, 1% triton X-100) supplemented with protease and phosphatase inhibitors. Protein concentrations were determined to ensure that each well was loaded with 5 μg of total proteins. Protein samples were resolved on SDS-PAGE gels and subjected by immunoblot with the following antibodies: Pggt1b (Sigma HPA030646), npRap1a (Santa Cruz sc1482), Phospho-Akt S473 (Cell Signaling, 4060), phosphor-Gsk3β Ser9 (Cell Signaling 9322), phosphor-Erk (Cell Signaling, 9101), phosphor-Stat3 Tyr705 (Cell Signaling, 9145), phosphor-Smad2 (Cell Signaling 3108), phosphor-Smad3 (Cell Signaling, 9520), and β-Actin-HRP (Sigma, A3854).

Swan G., Geng J., Park E., Ding Q., Zhou J., Walcott C., Zhang J.J., Huang H.I., Hammer G.E, & Wang D. (2021). A Requirement of Protein Geranylgeranylation for Chemokine Receptor Signaling and Th17 Cell Function in an Animal Model of Multiple Sclerosis. Frontiers in Immunology, 12, 641188.

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