Polycystin 1 ct2741

The following primary antibodies were used for Western blot analysis: Polycystin‐2 (sc‐10376 Santa Cruz Biotechnology), p70‐S6K (sc‐230 Santa Cruz Biotechnology), phospho‐p70‐S6K (sc‐8416 Santa Cruz Biotechnology), phospho‐mTOR (5536 CST), phospho‐4E‐BP1 (2855 CST), PTEN (9559 CST), Akt (9272 CST), phospho‐Akt (9271 CST), actin (MAB1501 Millipore), polycystin‐1 CT2741 (kindly provided by the Baltimore Polycystic Kidney Disease Research and Clinical Core Center), mTOR (701483 Thermo Fisher Scientific), 4EBP1 (AHO1382 Thermo Fisher Scientific), JAK2 (ab37226 Abcam), phospho‐JAK2 (ab32101 Abcam). The following secondary antibodies were used: goat anti‐mouse IgG HRP‐conjugate (AP124P Millipore), goat anti‐rabbit IgG HRP‐conjugate (AP132P Millipore), donkey anti‐goat IgG HRP‐conjugate (A00178 GenScript). The IgPKD1 inhibitory antibody was a generous gift from Dr O. Ibraghimov‐Beskrovnaya and H. Husson (Genzyme Co., Boston).

Cell culture media and all tissue culture reagents were obtained from Gibco (ThermoFisher Scientific) and Biosera. For immunohistochemistry, the following reagents were used: Dako Real Envision Detection System, peroxidase/DAB1, rabbit/mouse (Dako). The following primary antibodies were employed for Western blot analysis: polycystin‐2 (sc‐10376, Santa Cruz Biotechnology), p70S6K (sc‐230, Santa Cruz Biotechnology), phospho(p)‐p70S6K (sc‐8416, Santa Cruz Biotechnology), phospho(p)‐mTOR (5536, CST), phospho(p)‐4EBP1 (2855, CST), PTEN (9559,CST), AKT (9272,CST), phospho(p)‐AKT (9271, CST), actin (MAB1501, Millipore), polycystin‐1 CT2741 (kindly provided by the Baltimore Polycystic Kidney Disease Research and Clinical Core Center), mTOR (701483, Thermo Fisher Scientific), anti‐PC1 7E12 (sc‐130554, Santa Cruz Biotechnology) against the extracellular N‐terminal leucine‐rich domain, anti‐PC2 (sc‐28331, Santa Cruz Biotechnology) and anti‐PC2 (PAB2306, Abnova). The following secondary antibodies were used: goat anti‐mouse IgG HRP conjugate (AP124P, Millipore) and goat anti‐rabbit IgG HRP conjugate (AP132P, Millipore). The inhibitory IgPKD1 antibody (blocking the extracellular N‐terminal leucine‐rich domain) was a generous gift from Dr. O. Ibraghimov‐Beskrovnaya and H. Husson (Genzyme), and the PI3K inhibitor was obtained from Cayman, USA.