Mouse anti flag

Manufactured by Abcepta

Sourced in United States

Mouse anti-FLAG is a monoclonal antibody that specifically binds to the FLAG epitope tag. It is commonly used in research applications to detect and purify proteins that have been engineered to express the FLAG tag.

Automatically generated - may contain errors

Lab products found in correlation

2 protocols using mouse anti flag

Histone Modification and Signaling Pathway Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols

Cells were harvested and lysed in RIPA buffer with protease inhibitors and 2 μM PMSF. Protein extracts were boiled in SDS sample buffer for 5 min, loaded directly onto a 4–12% SDS gel, transferred onto nitrocellulose membranes (Bio-Rad), blocked with 5% milk, and incubated with corresponding primary and secondary antibodies using standard protocols. The following antibodies were used: anti-H3K4me1 (Cat # 07-436, 1:2000 dilution), anti-H3K4me2 (Cat # 07-030, 1:4000 dilution), anti-H3K4me3 (Cat # 07-473, 1:4000 dilution), anti-H3K9me1 (Cat # 07-450, 1:2000 dilution), anti-H3K9me2 (Cat # 07-441, 1:3000 dilution), anti-H3K9me3 (Cat # 07-442, 1:3000 dilution), anti-H3K27me1 (Cat # 07-448, 1:2000 dilution), anti-H3K27me2 (Cat # 07-452, 1:4000 dilution), and anti-H3K27me3 (Cat # 07-449, 1:4000 dilution) from Millipore; rabbit anti-Jmjd3 (Cat # ab1022a, 1:500 dilution) from Abgent; and mouse anti-FLAG (1:5000 dilution), anti-HRP-FLAG (1:5000 dilution), and anti-β-actin (1:5000 dilution) from Sigma; anti-Smad1 (Cat # 6944, 1:500 dilution), anti-Smad2 (Cat # 5339, 1:500 dilution) and anti-Smad3 (Cat # 9523, 1:500 dilution) from Cell Signaling; and anti-Ash2L (Cat # ab50699, 1:500 dilution) from Abcam.

Li Q., Zou J., Wang M., Ding X., Chepelev I., Zhou X., Zhao W., Wei G., Cui J., Zhao K., Wang H.Y, & Wang R.F. (2014). Critical role of histone demethylase Jmjd3 in the regulation of CD4+ T cell differentiation. Nature communications, 5, 5780.

+ Open protocol

+ Expand

Immunostaining of Flag-tagged FTH1 in SK-N-SH Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

To indirectly determine the expression levels of FTH1 by immunostaining, Flag-expressing SK-N-SH-FTH1 cells were treated under the same conditions as those of the western blot analysis. After treatment, the cells were rinsed with phosphate-buffered saline (PBS), fixed with 4% paraformaldehyde, and thoroughly washed. The samples were permeabilized with 1% Triton X-100 in PBS, blocked with 5% BSA at 37°C for 30 min, and incubated overnight at 4°C with a primary antibody against Flag (mouse anti-Flag 1:200; Abgent, San Diego, CA, USA). The cells were then washed with PBS and incubated in the dark at room temperature with the secondary antibody (Cy3-conjugated anti-mouse, 1:1,000; Beyotime, Nanjing, Jiangsu, China). Subsequently, 4, 6-diamidino-2-phenylindole (DAPI; Beyotime, Nanjing, Jiangsu, China) was used to counterstain cell nuclei. After mounting coverslips, the samples were imaged using a fluorescence microscope (Nikon, Tokyo, Japan).

He X., Cai J., Li H., Liu B., Qin Y., Zhong Y., Wang L, & Liao Y. (2016). In Vivo magnetic resonance imaging of xenografted tumors using FTH1 reporter gene expression controlled by a tet-on switch. Oncotarget, 7(48), 78591-78604.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!