Normal mice igg1 kappa monoclonal

Poly(D,L-lactic-co-glycolic acid) (PLGA7510; M W , 10,000) was purchased from Wako Pure Chemical Industries, Ltd. (Osaka, Japan). Human cyclin B1-speci c siRNA, which was modi ed with a thiol group at the 3 end of the sense or antisense strand, was purchased from Thermo Fisher Scienti c, Inc. (Waltham, MA, USA). The siRNA sequences were as follows: cyclin B1 siRNA sense: 5 -GGC GAA GAU CAA CAU GGC ATT-3 ; and cyclin B1 siRNA antisense: 5 -UGC CAU GUU GAU CUU CGC CTT-3 (17). Negative control siRNA was also purchased from Thermo Fisher Scienti c, Inc. (Waltham). Fetal calf serum (FCS) and Roswell Park Memorial Institute (RPMI) 1640 were purchased from Gibco (Cergy-Pontoise, France). Antihuman CD71 monoclonal antibody, phycoerythrin (PE) anti-human CD71 antibody (Clone: OKT9) and were purchased from Thermo Fisher Scienti c Inc. Normal mice IgG1 kappa monoclonal, which was used as the control IgG, was purchased from Abcam (ab91353, Cambridge, UK). A Pierce IgG F(ab )2 preparation kit was purchased from Thermo Fisher Scienti c Inc. Cell Counting Kit-8 (CCK-8) reagent was purchased from Dojindo Laboratories (Tokyo, Japan). Alexa488-labeled siRNA was purchased from Thermo Fisher Scienti c, Inc. (Waltham). Cy5-labeled siRNA was purchased from Nippon Gene (Toyama, Japan). FITC-labeled PLGA was synthesized by the NARD Institute, Ltd. (Amagasaki, Japan).