Murine fc blocking antibody 2.4g2

Manufactured by Cytek Biosciences

The Murine Fc-blocking antibody (2.4G2) is a laboratory reagent used to block Fc receptors in murine cell samples. It is designed to prevent non-specific binding of antibodies to Fc receptors, which can interfere with the accurate detection and analysis of target molecules.

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Lab products found in correlation

Fetal bovine serum (fbs), by Atlanta Biologicals (2 mentions) Histopaque 1077, by Merck Group (2 mentions) Histopaque 1119, by Merck Group (2 mentions) Mouse igg alkaline phosphatase, by Abcam (2 mentions) Cd11b, by Cytek Biosciences (2 mentions) Paraformaldehyde (pfa), by Electron Microscopy Sciences (2 mentions) Neutrophil elastase, by Abcam (1 mentions) H3cit, by Abcam (1 mentions) Baclight green bacterial stain, by Thermo Fisher Scientific (1 mentions) Lactoferrin, by Abcam (1 mentions) Celltrace violet, by Thermo Fisher Scientific (1 mentions) Cellmask deep red, by Thermo Fisher Scientific (1 mentions) Dnase 1, by Merck Group (1 mentions) Celltracker orange, by Thermo Fisher Scientific (1 mentions) Alexa fluor 488 streptavidin, by BioLegend (1 mentions) Dulbecco s modified eagle s medium, by Thermo Fisher Scientific (1 mentions) Helix np blue, by BioLegend (1 mentions) Cytochalasin d, by Cayman Chemical (1 mentions) Lympholyte, by Cedarlane (1 mentions) Rotenone, by Merck Group (1 mentions)

2 protocols using murine fc blocking antibody 2.4g2

Comprehensive Analysis of Neutrophil Biology

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Antibodies specific for Ly6G, F4/80, CD35, and rabbit immunoglobulin G (IgG) were from BioLegend; CD11b was from Tonbo; CD63 was from BD Biosciences; and MPO, H3Cit, lactoferrin, PR3, neutrophil elastase, Fas, dsDNA, and mouse IgG alkaline phosphatase were from Abcam. Streptavidin Alexa Fluor 488 was from BioLegend. Live/dead stain and DHR123, CellTracker Orange, CellTrace Violet, CellMask Deep Red, and BacLight Green Bacterial Stain were from Invitrogen; Helix NP Blue was from BioLegend. Murine Fc-blocking antibody (2.4G2) was from Tonbo. PMA and DNase I were ordered from Sigma-Aldrich. Cytochalasin D was purchased from Cayman Chemical. Paraformaldehyde was from Electron Microscopy Sciences. The neutrophil elastase activity kit was purchased from Abcam. Histopaque 1119 and Histopaque 1077 were from Sigma-Aldrich; Lympholyte was from Cedarlane Laboratories. Dulbecco’s modified Eagle’s medium (DMEM) and phosphate-buffered saline (PBS) that were from Gibco and fetal bovine serum (FBS) from Atlanta Biologicals were used for all tissue culture.

Monteith A.J., Miller J.M., Maxwell C.N., Chazin W.J, & Skaar E.P. (2021). Neutrophil extracellular traps enhance macrophage killing of bacterial pathogens. Science Advances, 7(37), eabj2101.

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Multiparametric Analysis of Neutrophil Activation

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Antibodies specific for Ly6G, CD3, CD19, Ly6C, CD15, CD16, rabbit IgG, and annexin V were from Biolegend; CD11b was from Tonbo; CD63 was from BD Biosciences; myeloperoxidase, histone citrulline, dsDNA, and mouse IgG-alkaline phosphatase were from Abcam; S100A9 (murine and human) was from Cell Signaling. Anti-nucleosome antibody (PL2–3) was a gift from Dr. B. Vilen. Streptavidin Alexa 488 was from Biolegend. Live/dead stain, mitoSOX, mitoTracker, and BacLight® Red Bacterial Stain were from Invitrogen; Helix NP Blue (Sytox) was from Biolegend. Murine Fc-blocking antibody (2.4G2) was from Tonbo; human Fc-blocking antibody was from BD Biosciences. Paraformaldehyde (PFA) was from Electron Microscopy Sciences. Rotenone was from Sigma. Histopaque® 1119 and Histopaque® 1077 were from Sigma. Dulbecco’s Modified Eagle’s Medium (DMEM) and phosphate buffered saline (PBS) was from Gibco and fetal bovine serum (FBS) from Atlanta biologicals were used for all tissue culture.

Monteith A.J., Miller J.M., Williams J.M., Voss K., Rathmell J.C., Crofford L.J, & Skaar E.P. (2021). Altered mitochondrial homeostasis during systemic lupus erythematosus impairs NET formation rendering neutrophils ineffective at combating Staphylococcus aureus. Journal of immunology (Baltimore, Md. : 1950), 208(2), 454-463.

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