The lung cell lines (WI-38, A549, NCI-H1573, NCI-H661, NCI-H460, NCI-H1975, NCI-H1563, and NCI-H1299) and human lung fibroblasts (WI-38) cells were purchased from American Type Culture Collection (Manassas, VA). WI38 cells were cultured in Minimum Essential Medium (Invitrogen, Carlsbad, CA), A549 cells were cultured in F12 Kaighn’s Medium (Invitrogen, Carlsbad, CA) and NCI-H1573, NCI-H661, NCI-H460, NCI-H1975, NCI-H1563, and NCI-H1299 cells were cultured in RPMI 1640 (Invitrogen, Carlsbad, CA). All media were supplemented with 10% heat-inactivated fetal bovine serum (Invitrogen, Carlsbad, CA), 100 U/ml penicillin and 100 μg/mL streptomycin (Invitrogen, Carlsbad, CA). The cultures were incubated at 37°C in 5% CO2/95% humidified air. In all cases, treatment was done in basal medium supplemented with 5% heat-inactivated fetal bovine serum.
Nci h1573
Manufactured by Thermo Fisher Scientific
Sourced in United States
The NCI-H1573 is a cell line derived from human non-small cell lung carcinoma. It is commonly used in research and development applications. This product's core function is to provide a standardized cell model for in vitro studies.
Automatically generated - may contain errors
Lab products found in correlation
Penicillin, by Thermo Fisher Scientific (2 mentions)
Streptomycin, by Thermo Fisher Scientific (2 mentions)
F12 kaighn s medium, by Thermo Fisher Scientific (2 mentions)
Nci h1975, by Thermo Fisher Scientific (2 mentions)
Nci h1975, by American Type Culture Collection (2 mentions)
Nci h1573, by American Type Culture Collection (2 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Minimum essential medium (mem), by Thermo Fisher Scientific (1 mentions)
Nci h460, by Thermo Fisher Scientific (1 mentions)
Nci h661, by Thermo Fisher Scientific (1 mentions)
Rpmi 1640, by Thermo Fisher Scientific (1 mentions)
Wi 38, by American Type Culture Collection (1 mentions)
Nci h460, by American Type Culture Collection (1 mentions)
Nci h1563, by American Type Culture Collection (1 mentions)
Nci h661, by American Type Culture Collection (1 mentions)
Neomycin, by Thermo Fisher Scientific (1 mentions)
Nci h1299, by Thermo Fisher Scientific (1 mentions)
Rpmi 1640 medium, by Thermo Fisher Scientific (1 mentions)
Nci h1299, by American Type Culture Collection (1 mentions)
2 protocols using nci h1573
1
Lung Cell Lines and Fibroblasts Culture
2
Culturing Human Lung Cancer Cell Lines
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Human lung cancer NCI-H1573, A549, NCI-H1299, and NCI-H1975 cells were purchased from American Type Culture Collection (ATCC, Manassas, VA, USA). A549 cells were routinely cultured in F12 Kaighn’s medium (Invitrogen, Carlsbad, CA, USA), and NCI-H1573, NCI-H1299, and NCI-H1975 were cultured in RPMI 1640 medium (Invitrogen, Carlsbad, CA, USA). The media used for the routine cell culture were supplemented with 10% (v/v) fetal bovine serum (FBS) and a 1% (w/v) combination of penicillin, streptomycin, and neomycin (Invitrogen, Carlsbad, CA, USA). The cultures were incubated in 75 cm2 vented culture flasks at 37 °C in 5% CO2/95% humidified air. The cells were trypsinized after they were 80–90% confluence and seeded onto the appropriate well plates.
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