Staining for LacZ expression was performed as previously described [47] on frozen sections and counterstained with Nile Red. Immunofluorescence experiments were performed after citrate based antigen retrieval. Primary antibodies were ZO-1 (Invitrogen cat# 339100), Occludin (BD Transduction cat# 611090), Claudin-1 (ABCAM cat# ab15098), cytokeratin14 - LLOO2 (ABCAM cat # ab7800), keratin10 (Covance PRB-159P), keratin6 (Covance cat # PRB-169P), Ecadherin (Life Technologies, 13-1900), phospho-histone H3 (Cell Signalling, #9708), PCNA (Santa Cruz Biotechnology sc-9857), CLDN1 (Santa Cruz Biotechnology, sc-81796), Keratin 76 (Sigma-Aldrich HPA019696) and Keratin 76 (Sigma-Aldrich HPA019656), Filaggrin (FLG- Covance PRB-417P), FASN (Santa Cruz Biotechnology, sc-48357), and Melan-A (MEL-A, Santa Cruz Biotechnology, sc-20032). All secondary antibodies were AlexaFluor conjugated (Invitrogen).
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