Plasma cfDNA extraction and bisulfite conversion were performed following the manufacturer’s instructions of the mSEPT9 assay (BioChain (Beijing) Science and Technology, Inc., Beijing, China). One PCR was performed for each subject on an ABI 7500 Fast Dx Real Time PCR device (Life Technologies, Thermo Fisher Technology (China) Co., LTD, Shanghai, China). ACTB was used as an internal reference to assess the integrity of each sample. The validity of mSEPT9 test results for each sample was determined on the basis threshold count (Ct) values of ACTB, the positive and negative controls. Plasma AFP and PIVKA-II levels were measured using the corresponding commercial kits with the Abbott ARCHITECT i2000SR chemiluminescence immunoanalyzer, according to manufacturer’s instructions (Abbott Laboratories; Chicago, IL, USA).
Abi 7500 fast dx real time pcr device
Manufactured by Thermo Fisher Scientific
Sourced in China, United States
The ABI 7500 Fast Dx Real Time PCR device is a laboratory instrument used for performing real-time polymerase chain reaction (PCR) analysis. It is designed to amplify and detect specific nucleic acid sequences in a sample.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using abi 7500 fast dx real time pcr device
1
Circulating Tumor DNA Extraction and Analysis
2
Septin9 DNA methylation detection
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Epi proColon 2.0 CE is a qualitative assay for the real time (RT) polymerase chain reaction (PCR) detection of methylated Septin9 DNA in bisulfite converted DNA from human plasma samples. The assay carried out in this study followed instructions and procedures in the instructions for use of Epi proColon 2.0 CE. The assay is comprised of the Epi proColon Plasma Quick Kit (M5-02-001), the Epi proColon Sensitive PCR Kit (M5-02-002), and the Epi proColon Control Kit (M5-02-003) (Epigenomics AG, Berlin, Germany). The 2/3 algorithm was used for data interpretation in this study. All kits were provided by BioChain (Beijing) Science and Technology, Inc. Beijing, China. The assays were performed using the ABI 7500 Fast Dx Real Time PCR device (Life Technologies, Applied Biosystems 7500/7500 Fast Real-Time PCR System, New York, USA). Assays were performed in 3 Chinese hospitals involved in this study by trained technicians.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!