Anti ku70 a9

Manufactured by Santa Cruz Biotechnology

Anti-KU70 (A9) is a laboratory antibody product provided by Santa Cruz Biotechnology. It is designed to detect the KU70 protein, which is a component of the DNA repair mechanism in cells. The antibody can be used for various research applications, such as Western blotting, immunoprecipitation, and immunohistochemistry, to study the expression and localization of the KU70 protein.

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Lab products found in correlation

Imagequant las 4000, by GE Healthcare (1 mentions) Anti p21 c 19, by Santa Cruz Biotechnology (1 mentions) Anti gfp, by Santa Cruz Biotechnology (1 mentions) Anti p53 do 1, by Santa Cruz Biotechnology (1 mentions) Mouse monoclonal anti β actin antibody, by Merck Group (1 mentions) Clarity western ecl blotting substrate, by Bio-Rad (1 mentions) Anti ha 12ca5, by Santa Cruz Biotechnology (1 mentions)

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Anti-Ku70 (13 citations)

2 protocols using anti ku70 a9

Western Blot Analysis of Protein Expression

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For Western blot analysis, samples were lysed in Laemmli buffer. Antibodies used were anti-p21 (c-19; Santa Cruz Biotechnology) anti-polη (H-300; Santa Cruz Biotechnology), anti-KU70 (A9; Santa Cruz Biotechnology), and anti-GFP (Santa Cruz Biotechnology). Secondary antibodies (Sigma) and ECL detection (Amersham GE Healthcare, Milwaukee, Wisconsin) were used according to the manufacturers’ instructions. Western blot images were taken with Image QuantLAS4000 (GE Healthcare), which allows capture and quantification of images within a linear range. These images were then quantified with the ImageJ software when indicated.

Mansilla S.F., Bertolin A.P., Bergoglio V., Pillaire M.J., González Besteiro M.A., Luzzani C., Miriuka S.G., Cazaux C., Hoffmann J.S, & Gottifredi V. (2016). Cyclin Kinase-independent role of p21CDKN1A in the promotion of nascent DNA elongation in unstressed cells. eLife, 5, e18020.

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Western Blot Antibody Characterization

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Anti-p53 (DO-1) (sc-126, Santa Cruz Biotechnology), anti-Ku70 (A-9) (sc-5309, Santa Cruz Biotechnology), anti-HA (12CA5) (sc: 57592, Santa Cruz Biotechnology), anti-acetyl-H4 (Upstate) antibodies. Filters were reprobed with anti-β-actin mouse monoclonal antibody (Sigma-Aldrich, 63103 USA) or anti-Sp1 mouse monoclonal antibody (Santa Cruz Biotechnology) to normalize respectively cytoplasmic or nuclear protein levels. Anti-H4 (F-9) (sc: 25260, Santa Cruz Biotechnology) mouse monoclonal antibody was used as internal control for acetylated H4. Filters were developed using an enhanced chemiluminescence system (Bio-Rad Clarity Western ECL Blotting Substrates).

Pucci S., Polidoro C., Greggi C., Amati F., Morini E., Murdocca M., Biancolella M., Orlandi A., Sangiuolo F, & Novelli G. (2019). Pro-oncogenic action of LOX-1 and its splice variant LOX-1Δ4 in breast cancer phenotypes. Cell Death & Disease, 10(2), 53.

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