Human HEK293T (CRL-11268) and Madin-Darby Canine Kidney (MDCK) cells (ATCC, CRL-2936) were maintained in Dulbecco's Minimal Essential Medium (DMEM) (Gibco) supplemented with 10% fetal bovine serum (Gibco), 100 units/mL penicillin, and 100 µg/mL streptomycin (Invitrogen). Authentication and test for the free of mycoplasma were performed with MycAwayTM one-step mycoplasma detection kit (Yeasen). Astrocytes were purchased from Cellapy (CA2315106) and cultured in NeuroEasy maintenance medium (Cellapy). Human embryonic stem cells (hESCs) were obtained from Harvard Stem Cell Institute. hESCs were routinely checked for pluripotent, normal karyotype, mycoplasma free and cultured in feeder-free conditions on Matrigel-coated plates with Essential 8 medium (GIBCO) and passaged with TrypLETM express (GIBCO). 10 μM Peramivir (M3222, AbMole BioScience), 1 μM Nucleozin (A3670, Apexbio), 50 μM PYC-12, 20 μM RO3306, 10 μM WH-L50B, 20 ng/mL BDNF (450-02, PeproTech), 20 ng/mL GDNF (450-10, PeproTech), 20 ng/mL NT3 (450-03, PeproTech) were used in this study.
Nucleozin
Manufactured by Apexbio
Nucleozin is a laboratory instrument designed for nucleic acid extraction and purification. It utilizes a proprietary technique to efficiently isolate DNA, RNA, and other nucleic acids from a variety of sample types. The core function of Nucleozin is to provide a reliable and consistent method for extracting high-quality nucleic acids for downstream applications.
Automatically generated - may contain errors
Lab products found in correlation
Fetal bovine serum (fbs), by Thermo Fisher Scientific (2 mentions)
Penicillin, by Thermo Fisher Scientific (2 mentions)
Streptomycin, by Thermo Fisher Scientific (2 mentions)
Tryple express, by Thermo Fisher Scientific (2 mentions)
Mycaway one step mycoplasma detection kit, by Yeasen (2 mentions)
Neuroeasy maintenance medium, by Cellapy (2 mentions)
Peramivir, by AbMole (2 mentions)
Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (2 mentions)
Essential 8 medium, by Thermo Fisher Scientific (2 mentions)
Brain derived neurotrophic factor (bdnf), by Thermo Fisher Scientific (2 mentions)
2 protocols using nucleozin
1
Cell Culture and Treatment Conditions
2
Cell Culture Protocols for Research
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Human HEK293T (CRL-11268) and Madin-Darby Canine Kidney (MDCK) cells (ATCC, CRL-2936) were maintained in Dulbecco's Minimal Essential Medium (DMEM) (Gibco) supplemented with 10% fetal bovine serum (Gibco), 100 units/mL penicillin, and 100 µg/mL streptomycin (Invitrogen). Authentication and test for the free of mycoplasma were performed with MycAway TM one-step mycoplasma detection kit (Yeasen).
Astrocytes were purchased from Cellapy (CA2315106) and cultured in NeuroEasy maintenance medium (Cellapy). Human embryonic stem cells (hESCs) were obtained from Harvard Stem Cell Institute. hESCs were routinely checked for pluripotent, normal karyotype, mycoplasma free and cultured in feeder-free conditions on Matrigel-coated plates with Essential 8 medium (GIBCO) and passaged with TrypLE TM express (GIBCO). 10 µM Peramivir (M3222, AbMole BioScience), 1 µM Nucleozin (A3670, Apexbio), 50 µM PYC-12, 20 µM RO3306, 10 µM WHL-50B, 20 ng/mL BDNF (450-02, PeproTech), 20 ng/mL GDNF (450-10, PeproTech), 20 ng/mL NT3 (450-03, PeproTech) were used in this study.
Astrocytes were purchased from Cellapy (CA2315106) and cultured in NeuroEasy maintenance medium (Cellapy). Human embryonic stem cells (hESCs) were obtained from Harvard Stem Cell Institute. hESCs were routinely checked for pluripotent, normal karyotype, mycoplasma free and cultured in feeder-free conditions on Matrigel-coated plates with Essential 8 medium (GIBCO) and passaged with TrypLE TM express (GIBCO). 10 µM Peramivir (M3222, AbMole BioScience), 1 µM Nucleozin (A3670, Apexbio), 50 µM PYC-12, 20 µM RO3306, 10 µM WHL-50B, 20 ng/mL BDNF (450-02, PeproTech), 20 ng/mL GDNF (450-10, PeproTech), 20 ng/mL NT3 (450-03, PeproTech) were used in this study.
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