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Rabbit anti eef2k

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Rabbit anti eEF2k is a primary antibody that recognizes the eukaryotic elongation factor 2 kinase (eEF2k) protein. eEF2k is a serine/threonine protein kinase that phosphorylates and inactivates the eukaryotic elongation factor 2 (eEF2), thereby regulating protein synthesis.

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Lab products found in correlation

Mouse anti gapdh, by Santa Cruz Biotechnology (1 mentions) Sc 365062, by Cell Signaling Technology (1 mentions) Rabbit anti cyclin d1, by Cell Signaling Technology (1 mentions) Mouse anti actin, by Developmental Studies Hybridoma Bank (1 mentions) Rabbit anti phospho eef2 thr56, by Cell Signaling Technology (1 mentions) Mouse anti c myc, by Cell Signaling Technology (1 mentions)

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EEF2K (12 citations)

2 protocols using rabbit anti eef2k

1

Immunoblotting with GAPDH and eEF2k

Cited 1 time
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We performed immunoblots as described previously14 (link). Additional antibodies used were mouse anti gapdh (1:2000, Santa Cruz Biotechnology, sc-365062) and rabbit anti eEF2k (1:800, Cell Signaling Technology; 3692).
Parihar M., Dodds S.G., Hubbard G., Javors M., Strong R., Hasty P, & Sharp Z.D. (2020). Rapamycin extends life span in ApcMin/+ colon cancer FAP model. Clinical colorectal cancer, 20(1), e61-e70.
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2

Optimized Cell Lysis and Western Blotting

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For coomassie blue staining, 1 × 104 of cells were collected and lysed in 100 µl of lysis buffer. 10 µl of lysates were loaded. For western blots, 20 µg of total proteins were loaded. All primary antibodies were used at 1:500 dilution: rabbit anti-eEF2K (#3692, Cell Signaling Technology), rabbit anti-phospho-eEF2 (Thr56) (#2331, Cell Signaling Technology), rabbit anti-Cyclin D1 (#2922, Cell Signaling Technology), 4EBP1, p-dIF4E, rabbit anti-Tubulin (#2148, Cell Signaling Technology), rabbit anti-Collagen Type I Alpha 1 (COL1a1) (PAA350HU02, Cloud Clone Corp.), mouse anti-C-myc (9E10) (generously provided by Dr. Linda Penn), mouse anti-CDC2 (CDK1) (#9116, Cell Signaling Technology), and mouse anti-Actin (JLA20, Developmental Studies Hybridoma Bank) antibodies.
Ju Y., Ben-David Y., Rotin D, & Zacksenhaus E. (2021). Inhibition of eEF2K synergizes with glutaminase inhibitors or 4EBP1 depletion to suppress growth of triple-negative breast cancer cells. Scientific Reports, 11, 9181.
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