Procedures of isolation and staining of erythroid populations and EBI macrophages were described previously (Chen et al., 2009 (link); Flygare et al., 2011 (link); Yang et al., 2021 (link)). The following antibodies were used for flow cytometric analysis: F4/80 (BM8; BioLegend), Vcam1 (MVCAM.A; BioLegend), CD169 (3D6.112; BioLegend), TER-119 (Ter119; BD Bioscience), CD44 (IM7; BD Bioscience), Sirpα (P84; BioLegend), CD71(C2; BD Bioscience), c-Kit (2B8; Biolegend), Sca-1 (D7; BioLegend), CD4 (RM4-5; BD Bioscience), CD8 (53-6.72; BD Bioscience), B220 (RA3-6B2; BD Bioscience), Gr-1 (RB6-8C5; BD Bioscience), and Mac-1 (M/70; BD Bioscience). For measuring ATP content and proliferation, early erythroblasts were sorted in 96-well plates and measured for luminescence via CellTiter-Glo 2.0 Assay (Promega). Flow cytometric cell sorting was performed using Aria II (BD Biosciences) and analyzed using FlowJo software package (Tree Star).
Cd71 c2
Manufactured by BD
CD71(C2) is a laboratory equipment product. It serves as a core function of its intended use, without any further interpretation or extrapolation.
Automatically generated - may contain errors
Lab products found in correlation
Cd4 rm4 5, by BD (2 mentions)
Gr 1 rb6 8c5, by BD (2 mentions)
B220 ra3 6b2, by BD (2 mentions)
F4 80 bm8, by BioLegend (1 mentions)
Cd169 3d6.112, by BioLegend (1 mentions)
C kit 2b8, by BioLegend (1 mentions)
Sca1 d7, by BioLegend (1 mentions)
Sirpα p84, by BioLegend (1 mentions)
Cd44 im7, by BD (1 mentions)
Aria 2, by BD (1 mentions)
Cd8 53 6.72, by BD (1 mentions)
Mac 1 m 70, by BD (1 mentions)
Celltiter glo 2.0 assay, by Promega (1 mentions)
Ter119 ly 76, by BD (1 mentions)
Cd127 a7r34, by Thermo Fisher Scientific (1 mentions)
Cd135 a2f10, by Thermo Fisher Scientific (1 mentions)
Sca1 ly 6a e, by BD (1 mentions)
Cd8 53 6, by BD (1 mentions)
7 aad, by Thermo Fisher Scientific (1 mentions)
Cd11b m1 70, by BD (1 mentions)
2 protocols using cd71 c2
1
Isolation and Staining of Erythroid Populations
2
Multicolor Flow Cytometry for Bone Marrow and Spleen Progenitors
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
For assessment of bone marrow and spleen progenitor cells by flow cytometry, single-cell suspensions of freshly prepared bone marrow and spleen were incubated with the following anti-mouse monoclonal antibodies: Ter119 (Ly-76, BD Biosciences), CD71 (C2, BD Biosciences), CD34 (RAM34, BD Biosciences), CD135 (A2F10, eBioscience), CD127 (A7R34, eBioscience), c-Kit (ACK2, eBioscience), SCA-1 (LY-6A/E, BD Biosciences), eFluor520 (eBioscience), and 7-AAD (eBioscience). Lineage (lin) staining included anti-mouse Ter119 (BD Biosciences), GR-1 (RB6-8C5, BD Biosciences), B220 (RA3-6B2, BD Biosciences), CD4 (RM4-5, BD Biosciences), CD8 (53–6.7, BD Biosciences), and CD11b (M1/70, BD Biosciences) antibodies [22 (link)–24 (link)]. Multicolor data acquisition for bone marrow progenitor subsets was performed on a FACSCanto II flow cytometer, and data were analyzed with FlowJo 7.6.1.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!