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2 protocols using anti lox 1 antibody

1

Anti-inflammatory Mechanisms of Ursolic Acid

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UA (purity > 98%) was purchased from Chengdu Herbpurify Co. Ltd. (Chendu, China). Hoechst 33342, N-acetyl cysteine (NAC), LPS (Escherichia coli serotype 055:B5, LPS), 5-(6)-carboxy-2′, 7′-dichlorodihydrofluorescein diacetate (DCFH2-DA), and BAY11-7082 were purchased from Sigma Aldrich (St. Louis, MO, USA). Antibodies for TLR4, MyD88 were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Antibodies for NF-κB p65, GAPDH, and Histone H3 were purchased from Cell Signaling Technology (Beverly, MA, USA). Anti-LOX-1 antibody was obtained from R&D (Minneapolis, MN, USA). The BCA protein kits were purchased from Thermo Fisher (Suwanee, GA, USA). SiRNAs for TLR4, MyD88 were purchased from Gene Pharma Company (Shanghai, China). Oil Red O Staining Kit and HE Staining Kit were obtained from Nanjing Jiancheng Bioengineering Research Institute (Nanjing, China).
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2

Oxidized LDL-Induced Inflammatory Signaling

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CPT (>95%) was purchased from SICHUAN WEIKEQI BIOLOGICAL TECHNOLOGY CO., LTD (Chengdu, China). TNF-α and Trizol were purchased from Sangon Biotech (Shanghai, China). 5-(6)-carboxy-2',7'-dichlorodihydrofluorescein diacetate (CM-DCFH2-DA) was purchased from Sigma (St. Louis, MO, USA). Antibodies for phospho-ERK (p-ERK), phospho-NF-κB p65 (p-p65) and actin antibodies were purchased from Cell Signaling Technology (Beverly, USA). Anti-LOX-1 antibody was obtained from R&D Systems (Minneapolis, USA). Diphenyleneiodonium chloride (DPI), rotenone (Rot), PD98059, BAY11-7082, and N-acetyl-L-cysteine (NAC) were purchased from Beyotime (Haimen, China). Primers and other materials for Real-Time PCR were purchase from Sangon Biotech (Shanghai, China) and TaKaRa Bio Group (Japan). BCA protein kit was purchased from Thermo Fisher (Rockford, USA). DiI-ox-LDL was purchased from Yiyuan Biotechnology (Guangzhou, China).
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