Smartbeam 3dtm

Manufactured by Bruker

Sourced in Germany

The Smartbeam 3DTM is a high-performance laser scanning electron microscope (SEM) from Bruker. It provides high-resolution, three-dimensional imaging capabilities for a wide range of applications.

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Lab products found in correlation

Fleximaging 5, by Bruker (2 mentions) Scils lab mvs 2018b, by Bruker (1 mentions) Rapiflex, by Bruker (1 mentions) Maldi tof tof ms, by Bruker (1 mentions) Smartbeam 2, by Bruker (1 mentions) Ultraflextreme, by Bruker (1 mentions)

2 protocols using smartbeam 3dtm

MALDI-MSI Protocol for AZD2858 Detection

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MALDI-MSI experiments were carried out in positive ion reflectron mode over a mass range of m/z 100 to 1000 using a MALDI rapifleX Tissuetyper MALDITOF/TOF MS (Bruker Daltonics, Bremen, Germany) equipped with a 10 kHz Smartbeam 3DTM Nd:YAG laser. Data collected on the rapifleX was at a spatial resolution of 50 µm, summing up 400 laser shots/raster position. FlexImaging 5.0 (Bruker Daltonics, Bremen, Germany) software was used for initial data analysis. Normalization, molecular image extraction, and regions of interest were defined in SCiLS Lab MVS 2018b (Bruker Daltonics, Bremen, Germany) software typically using a mass selection window of ± 0.05 Da. AZD2858 was detected as the protonated molecular ion (M + H+) at m/z 454.2.

Brüning-Richardson A., Shaw G.C., Tams D., Brend T., Sanganee H., Barry S.T., Hamm G., Goodwin R.J., Swales J.G., King H., Steele L., Morton R., Widyadari A., Ward T.A., Esteves F., Boissinot M., Mavria G., Droop A., Lawler S.E, & Short S.C. (2021). GSK-3 Inhibition Is Cytotoxic in Glioma Stem Cells through Centrosome Destabilization and Enhances the Effect of Radiotherapy in Orthotopic Models. Cancers, 13(23), 5939.

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MALDI-MSI Protocol for Quantitative Metabolomics

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MALDI-MSI experiments were carried out in negative reflectron mode over a mass range of m/z 60 to 1000 using a MALDI RapiFlex tissuetyper (lactate) or a UltrafleXtreme (glutamate) MALDI-TOF/TOF MS (Bruker Daltonics, Bremen, Germany) equipped with a 10kHz Smartbeam 3D TM and a 2 kHz, smartbeam-II™ Nd:YAG laser respectively. Data was collected on the RapiFlex was at a spatial resolution of 50 µm, summing up 500 laser shots/raster position. Data collection on the UltrafleXtreme was at a spatial resolution of 80 µm, summing up 300 laser shots /raster position. FlexImaging 5.0 (Bruker Daltonics, Bremen, Germany) software was used for initial data analysis. Normalization, molecular image extraction and regions of interest for lactate quantitation were defined in SCiLS Lab 2017b (Supplier ?) software typically using mass selection window of ± 0.05 Da. Glutamate quantitation was performed using msIQuant software 26 . Endogenous lactate, lactate 13 C1 (for normalization) lactate 13 C3 were detected at m/z 89.1, 90.1 and 92.1 respectively.

Swales J.G., Dexter A., Hamm G., Nilsson A., Strittmatter N., Michopoulos F., Hardy C., Morentin-Gutierrez P., Mellor M., Andren P.E., Clench M.R., Bunch J., Critchlow S.E, & Goodwin R.J.A. (2018). Quantitation of Endogenous Metabolites in Mouse Tumors Using Mass-Spectrometry Imaging. Analytical chemistry, 90(10).

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