2-Amino-2-(hydroxymethyl)-1,3-propanediol (Tris), Triton X-100, H2O2, NaCl, EDTA and NaOH were purchased from Merck Co. (Germany). Low melting point agarose (LMA), Na2HPO4, KCl and ethidium bromide were supplied by Sigma Co. (USA). Normal melting point agarose (NMA) was obtained from Cinnagen Co. (Iran). Roswell Park Memorial Institute (RPMI-1640), fetal bovine serum (FBS) and antibiotics were purchased from PAA Co. (Australia). Lovastatin was a gift from Amin Pharmaceutical Company (Iran). BLM was procured from Cell Pharm Co. (Germany).
Low melting point agarose lma
Manufactured by Merck Group
Sourced in United States, Germany
Low melting point agarose (LMA) is a type of agarose, a polysaccharide extracted from red seaweed. It has a lower melting and gelling temperature compared to standard agarose. LMA is commonly used in molecular biology and biochemistry applications that require a gel matrix with a lower melting point.
Automatically generated - may contain errors
Lab products found in correlation
Ethidium bromide, by Merck Group (2 mentions)
Na2hpo4, by Merck Group (2 mentions)
Potassium chloride (kcl), by Merck Group (2 mentions)
Triton x 100, by Merck Group (2 mentions)
Sodium hydroxide (naoh), by Merck Group (2 mentions)
Sodium chloride (nacl), by Merck Group (2 mentions)
Normal melting point agarose nma, by CinnaGen (2 mentions)
Normal melting point agarose nma, by Merck Group (2 mentions)
Ethylene diamine tetraacetic acid (edta), by Merck Group (1 mentions)
Nah2po4, by Merck Group (1 mentions)
2 7 dichlorofluoresce diacetate dcfh da, by Thermo Fisher Scientific (1 mentions)
Bromuconazole, by Merck Group (1 mentions)
Ethidium bromide etbr, by Merck Group (1 mentions)
Dimethyl sulfoxide (dmso), by Merck Group (1 mentions)
5 protocols using low melting point agarose lma
1
Cytotoxicity Assay of Lovastatin and BLM
2
Cytotoxicity Assay for HepG2 Cells
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DTIC and amifostine were respectively purchased from Medac Co. (Germany) and Medlmmune Pharma BV. (Poland). Tris, Triton X-100, H2O2, NaCl, ethylenediaminetetraacetic acid (EDTA), NaOH and NaH2PO4 were procured from Merck Co. (Germany). Low melting point agarose (LMA), Na2HPO4, KCl and ethidium bromide obtained from Sigma Co. (USA). Normal melting point agarose (NMA) was supplied by Cinnagen Co. (Iran), RPMI-1640, FBS and antibiotic were purchased from PAA Co. (Australia). HepG2 cells obtained from Pasture Institute (Iran).
3
Cytotoxicity Evaluation of Triflumuron and Fennel
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Triflumuron (TFM) and Fennel Essential Oil (FEO) were purchased from Sigma-Aldrich (St. Louis, MO, USA). 3-4,5-Dimethylthiazol-2-yl, 2,5-diphenyltetrazolium bromide (MTT), cell culture medium (MEM), foetal calf serum (FCS), phosphate buffer saline (PBS), trypsin-EDTA, penicillin and streptomycin mixture and L-glutamine (200 mM) were from GIBCO-BCL (UK). 2, 7-Dichlorofluoresce diacetate (DCFH-DA) was supplied by Molecular Probes (Cergy Pontoise, France). Low melting point agarose (LMA) and normal melting point agarose (NMA) were purchased from Sigma (St. Louis, MO, USA). Mouse anti-Hsp 70 (SP-810) was from stressgen (Canada), mouse monoclonal antibax and anti-bcl2 were from Invitrogen (Carlsbad, California, USA) and mouse anti -GAPDH (ab983) was from ABCAM. All other chemicals used were of analytical grade. All other chemicals used were of analytical grade.
4
Bromuconazole and Fluorescent Assays
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Bromuconazole (1-[[4-bromo-2-(2,4-dichlorophenyl) tetrahydro-2-furanyl] methyl]-1H-1,2,4-triazole); 2,4-Dinitro-phenylhydrazine (2,4-DNPH); 2,7-Dichloro uoresce diacetate (DCFH-DA); Low melting point agarose (LMA) and normal melting point agarose (NMA) were purchased from Sigma (Steinheim, Germany).
5
Comet Assay for DNA Damage
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BA and H2O2 were purchased from Sigma-Aldrich
(Germany). For cell culture, we used Dulbecco’s
Modified Eagle’s Medium (DMEM, Biological Industries,
Israel) and fetal calf serum (FCS, Sigma-
Aldrich, Germany). Dimethyl sulfoxide (DMSO)
was the product of Merck (Germany). The NR solution,
normal melting point agarose (NMA) and low
melting point agarose (LMA) were purchased from
Sigma-Aldrich (Germany). Sodium chloride (NaCl),
disodium ethylene diaminetetraacetic acid (Na2EDTA),
Triton-X 100, Tris and sodium sarcosinate
were purchased from Amresco (OH, USA). Ethidium
bromide (EtBr) was purchased from Sigma-Aldrich
(Germany) for fluorescent dying in the comet assay.
(Germany). For cell culture, we used Dulbecco’s
Modified Eagle’s Medium (DMEM, Biological Industries,
Israel) and fetal calf serum (FCS, Sigma-
Aldrich, Germany). Dimethyl sulfoxide (DMSO)
was the product of Merck (Germany). The NR solution,
normal melting point agarose (NMA) and low
melting point agarose (LMA) were purchased from
Sigma-Aldrich (Germany). Sodium chloride (NaCl),
disodium ethylene diaminetetraacetic acid (Na2EDTA),
Triton-X 100, Tris and sodium sarcosinate
were purchased from Amresco (OH, USA). Ethidium
bromide (EtBr) was purchased from Sigma-Aldrich
(Germany) for fluorescent dying in the comet assay.
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