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2 protocols using dylight 800 680 secondary antibodies

1

Comprehensive Protein Analysis Protocol

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Anti-AKT (pan), anti-p-AKT (S473), anti-p44/42 MAPK, and anti-p-p44/42 MAPK (pTEpY) were purchased from Cell Signaling. Anti-Smad3 and anti-p-Smad3 (S423/425) were from Abcam. Monoclonal anti-β-actin was from Sigma. Anti-F4/80 and SYBR Green PCR Master Mix were from Bio-Rad. PCR primers (Table 1) were designed with Primer Express software and purchased from IDT, Inc. Protease Inhibitor Mixture was from Calbiochem. The DyLight 800/680 secondary antibodies were purchased from Thermo Scientific.
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2

Immunological Reagents for Signaling Pathways

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Anti-TLR7 (against N-terminal for WB), anti-ATF4 and anti-Xbp1 were from Abcam. Anti-TLR7 (against C-terminal for WB) was purchased from Cell Signaling. Anti-TLR7 (against N-terminal) from Invitrogen, Anti-TLR7 (against C-terminal) and Anti-TLR7 (against C-terminal for IF) from Noves Biologicals. Anti-Pin1 and anti-ATF6 from Santa Cruz Biotechnology. SYBR Green PCR Master Mix was from Bio-Rad. R848, OVA and anti-β-actin were from Sigma. PCR primers were purchased from IDT, Inc. Protease Inhibitor Mixture was from Calbiochem. The DyLight 800/680 secondary antibodies and IMJECT Alum were purchased from Thermo Scientific. TaqMan Universial PCR Master Mix (for Rhinovirus) and TaqMan Gene Expression Assay (for SeV) were from Applied Biosystem. Sendai virus was from ATCC. Mouse TLR1–9 Agonist kit was from InvivoGen. Murine IL-5, mSCF and mFLT3L were from PeproTech. EDN (RNase2) ELISA kit, MBP (Prg2) ELISA kit and ECP ELISA kit were from MyBioSource. Anti-PRG2, anti-ECP and anti-EDN were from Biorbyt. LEGENDplx Mouse Proinflammatory Chemokine Panel was from Biolegend.
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