The following antibodies were used to detect protein expression: c-Abl (Cell signaling), ALK (Cell signaling), Raf-B (Santa Cruz), MET (Cell signaling), TrkB (abcam), Phospho-Stat1 (Y701; Cell signaling), Stat1 (Cell signaling), Phospho-Stat3 (Y705; Cell signaling), Phospho-AKT (S473 & T308; Cell signaling), Phospho-ERK1/2 (T202/Y204; Cell signaling), ERK1/2 (Cell signaling), Vinculin (Cell signaling), and GAPDH (Santa Cruz).
Raf b
Manufactured by Santa Cruz Biotechnology
Raf-B is a laboratory reagent used for research purposes. It functions as a serine/threonine-protein kinase that plays a role in the regulation of the mitogen-activated protein kinase (MAPK) signaling pathway. Raf-B is commonly used in cellular and molecular biology studies to investigate signal transduction processes.
Automatically generated - may contain errors
Lab products found in correlation
Rabbit antimouse, by Santa Cruz Biotechnology (2 mentions)
Hrp conjugated mouse anti rabbit, by Santa Cruz Biotechnology (2 mentions)
P mtor, by Cell Signaling Technology (2 mentions)
P erk, by Cell Signaling Technology (2 mentions)
β actin, by Merck Group (2 mentions)
C abl, by Santa Cruz Biotechnology (1 mentions)
Vinculin, by Santa Cruz Biotechnology (1 mentions)
Erk1 2, by Santa Cruz Biotechnology (1 mentions)
Phospho erk1 2, by Cell Signaling Technology (1 mentions)
Stat1, by Cell Signaling Technology (1 mentions)
Phospho stat1, by Cell Signaling Technology (1 mentions)
Phospho stat3, by Cell Signaling Technology (1 mentions)
Phospho akt, by Cell Signaling Technology (1 mentions)
Gapdh, by Santa Cruz Biotechnology (1 mentions)
3 protocols using raf b
1
Protein Expression Detection Assay
2
Quantitative Analysis of Protein Expression
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The frozen kidney of each rat was thawed, dissected into the cortex and medulla, and then homogenized in 100 mmol·L−1 potassium buffer (pH 7.25) containing 30% glycerol, 1 mmol·L−1 dithiothreitol, and 0.1 mmol·L−1 phenylmethylsulfonyl fluoride (15 (link)). Protein expression and phosphorylation were examined using Western blot analysis, as described previously (19 (link)). Antibodies against Raf-B (no. 5284, Santa Cruz), ERK (no. 4695, Cell Signaling Technology), p-ERK (no. 4376, Cell Signaling Technology), mTOR (no. 2983, Cell Signaling Technology), p-mTOR (no. 2971, Cell Signaling Technology), S6 (no. 2217, Cell Signaling Technology), and p-S6 (no. 2211, Cell Signaling Technology) were used. Secondary HRP-conjugated mouse antirabbit (no. 2357, Santa Cruz) and rabbit antimouse (no. 516102, Santa Cruz) antibodies were then used. Relative band intensities were quantified using ImageJ and normalized using β-actin (A2228; Sigma-Aldrich, St. Louis, MO) as an internal standard.
3
Kidney Protein Extraction and Analysis
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The frozen kidney of each rat was thawed, dissected into the cortex and medulla, and then homogenized in 100 mmol/L potassium buffer (pH 7.25) containing 30% glycerol, 1 mmol/L (which was not certified by peer review) is the author/funder. All rights reserved. No reuse allowed without permission.
dithiothreitol, and 0.1 mmol/L phenylmethylsulfonyl fluoride (14). Protein expression and phosphorylation were examined using western blot analysis, as described previously (18) .
Antibodies against Raf-B (#5284; Santa Cruz), ERK (#4695; Cell Signaling Technology), p-ERK (#4376; Cell Signaling Technology), mTOR (#2983; Cell Signaling Technology), p-mTOR (#2971; Cell Signaling Technology), S6 (#2217; Cell Signaling Technology), and p-S6 (#2211; Cell Signaling Technology) were used. Secondary HRP-conjugated mouse anti-rabbit (#2357; Santa Cruz) and rabbit anti-mouse (#516102; Santa Cruz) antibodies were then used. Relative band intensities were quantified using ImageJ and normalized using β-actin (A2228; Sigma-Aldrich, St.
Louis, MO, USA) as an internal standard.
dithiothreitol, and 0.1 mmol/L phenylmethylsulfonyl fluoride (14). Protein expression and phosphorylation were examined using western blot analysis, as described previously (18) .
Antibodies against Raf-B (#5284; Santa Cruz), ERK (#4695; Cell Signaling Technology), p-ERK (#4376; Cell Signaling Technology), mTOR (#2983; Cell Signaling Technology), p-mTOR (#2971; Cell Signaling Technology), S6 (#2217; Cell Signaling Technology), and p-S6 (#2211; Cell Signaling Technology) were used. Secondary HRP-conjugated mouse anti-rabbit (#2357; Santa Cruz) and rabbit anti-mouse (#516102; Santa Cruz) antibodies were then used. Relative band intensities were quantified using ImageJ and normalized using β-actin (A2228; Sigma-Aldrich, St.
Louis, MO, USA) as an internal standard.
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