The largest database of trusted experimental protocols

Cyan lx9 cytometer

Manufactured by Beckman Coulter
Sourced in France

The Cyan LX9 is a flow cytometry instrument designed for cell analysis. It is capable of detecting and quantifying various cell properties, including size, granularity, and the presence of specific surface markers. The Cyan LX9 utilizes multiple lasers and detectors to provide high-resolution data on the characteristics of individual cells within a sample.

Automatically generated - may contain errors

Lab products found in correlation

2 protocols using cyan lx9 cytometer

1

Mitochondrial Membrane Potential Dynamics in Prostate Cancer Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
LNCaP or PC3M cells were seeded on tissue culture dishes with cover glass bottom (FluoroDish, FD35, World Presicion Instruments, Inc.). Changes in mitochondrial membrane potential (ΔΨ) were imaged by means of the fluorescent ΔΨ-dependent lipophilic cationic dye tetramethyl-rhodamine ethyl ester (TMRE) which accumulates in active mitochondria. LNCaP and PC3M cells were stained with TMRE (0.1 µM, 10 min), treated with vehicle, CQ (20 μM) or FQ (20 μM) for 2 h and changes in intensity of TMRE signal were analyzed by confocal microscopy. To confirm the mitochondrial origin of the TMRE signal, at the end of the imaging protocol the cells were exposed to 5 µmol/L FCCP. TMRE fluorescence was excited by the 543 nm line of a 5 mW HeNe ion laser and the emitted fluorescence signal was captured at wavelengths above 560 nm.
Alternatively, ΔΨ was assessed using another ΔΨ-sensitive probe 3,3′-Dihexyloxacarbocyanine Iodide (DiOC6(3)) (318426, Aldrich). LNCaP or PC3M cells were treated with vehicle, CQ (15 μM) or FQ (15 μM) for 15 h, stained with DiOC6(3) (40 nM) for 15 min at 37 °C and analyzed by flow cytometry using Cyan LX9 cytometer (Beckman Coulter). Data analysis was performed using Summit 4.3 software (Beckman Coulter).
+ Open protocol
+ Expand
2

Cell Cycle Analysis of ORAI3 Knockdown

Check if the same lab product or an alternative is used in the 5 most similar protocols
MiaPaCa2 cells were seeded in 6-cm dishes in normal medium and transfected with either siCT or siORAI3. After 96 hours, cells were collected by trypsinization, centrifuged and washed with PBS and fixed overnight with 70% ice-cold ethanol at -20 °C. Fixed cells were treated with RNAse A (100 μg/ml) for 15 min at room temperature, and stained with a PBS-based solution containing Propidium Iodide (50 μg/ml). Cell cycle was analyzed with Cyan LX9 cytometer (Beckman Coulter, France) and data were processed with Summit 4.5 software (Beckmann Coulter, France).
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!