HEK293T, LnCap, and PC3 were obtained from ATCC and were propagated according to ATCC datasheets. LnCap-CR were generated by culturing LnCap cells in RPMI medium supplemented with 10% charcoal-stripped FBS (#S181F; Biowest) for more than 2 months. PrEC cells were purchased from Lonza (CC-2555) and propagated according to the manufacturer’s data sheet.
Lncap
Manufactured by Biowest
Sourced in United States, France
LNCaP is a human prostate cancer cell line. It is a commonly used model for studying prostate cancer biology and evaluating potential therapeutic agents.
Automatically generated - may contain errors
Lab products found in correlation
Rpmi 1640, by Biowest (4 mentions)
Lncap, by American Type Culture Collection (4 mentions)
Hek293t, by American Type Culture Collection (2 mentions)
Charcoal stripped fbs, by Biowest (1 mentions)
Cc 2555, by Lonza (1 mentions)
Hct116, by Biowest (1 mentions)
Ham s f12, by Biowest (1 mentions)
Dmem high glucose medium, by Biowest (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Docetaxel, by Merck Group (1 mentions)
Hek293t, by Biowest (1 mentions)
Rabbit anti parp1 h 250, by Santa Cruz Biotechnology (1 mentions)
Goat anti actin 1 19, by Santa Cruz Biotechnology (1 mentions)
F 12k nutrient mixture medium, by Thermo Fisher Scientific (1 mentions)
Mouse anti ha 11 16b12, by Fortrea (1 mentions)
Fetal bovine serum (fbs), by Biowest (1 mentions)
Du145, by American Type Culture Collection (1 mentions)
Penicillin, by Biowest (1 mentions)
Streptomycin, by Biowest (1 mentions)
L glutamine, by Biowest (1 mentions)
5 protocols using lncap
1
Cell Line Cultivation and Derivation
2
Culturing Prostate Cancer Cell Lines
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The PCa cell lines PC-3 (CLR-1435) and LNCaP (CRL-1740) were obtained from American Type Culture Collection (ATCC) and cultured as follows: LNCaP in RPMI 1640 (L0495, Biowest, Logan, UT, USA), PC-3 in F-12K (21127-022, Life Technologies, Carlsbad, CA, USA) plus 10% fetal bovine serum (FBS; S181P, Biowest) in a humidified incubator with a 5% CO2 atmosphere at 37ºC.
3
Cell Culture Conditions for Human Cancer Cell Lines
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The human colon cell lines, HT29 (ATCC Cat# HTB-38) and HCT116 (ATCC Cat# CCL-247), were grown in DMEM High-Glucose Medium (Biowest, Nuaillé, France), while the human prostate cell lines, PC3 (ATCC Cat# CRL-1435) and LNCaP (ATCC Cat# CRL-1740), were grown in Ham’s F12 (Biowest, Nuaillé, France) and RPMI 1640 (Biowest, Nuaillé, France), respectively. All media were supplemented with 2 mM glutamine, 100 IU/mL penicillin, 100 μg/mL streptomycin, and 10% fetal bovine serum (FBS, Gibco, Fisher Scientific Italia, Segrate (MI), Italia)). The cells were grown at 37 °C in a humified 5% CO2 atmosphere.
4
Prostate Cancer Cell Lines Characterization
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HEK293T, human prostate adenocarcinoma LNCaP, Du145 and PC3 cells lines were purchased from the American Type Culture Collection, Manassas, VA, USA. The Docetaxel resistant cell lines Du145 and PC3 were developed as previously described [31 (link)]. HEK293T were cultured in DMEM (BioWest, Nuaillé, France), Docetaxel sentitive and resistant Du145 cells and LNCaP cells were cultured in RPMI-1640 (BioWest) and PC3 DS and PC3 DR in F12K nutrient mixture medium (Thermo Fisher Scientific, Waltham, MA, USA). Culture media were supplemented with 10% fetal bovine serum (FBS), 2 mM l-glutamine, 100 U of penicillin/ml, 100 μg of streptomycin/ml, and 0.1 mM non-essential amino acids (all from BioWest). Docetaxel resistant cell lines were maintained with 2.5 nM of Docetaxel (Sigma-Aldrich, St. Louis, MO). Antibody to PTOV1 was produced and purified as previously described [8 (link), 9 (link)]. Additional antibodies were obtained from: goat anti-actin (I-19) (Santa Cruz Biotechnology, Santa Cruz, CA); mouse anti-HA.11 (16B12) (Covance, MA); mouse anti-Cyclin B1(V152) (Abcam, Cambridge, MA); rabbit anti-PARP1 (H-250) (Santa Cruz Biotechnology). Lentiviral vectors carrying short-hairpin RNA (shRNA, TRCN0000143905, TRCN0000140104 and TRCN0000139737) to PTOV1 were obtained from Sigma.
5
Prostate Cancer Cell Culture Conditions
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The prostate cancer cell lines PC-3 (CLR-1435) and Lncap (CRL-1740) were obtained from American Type Culture Collection (ATCC) and cultured as follows: Lncap in RPMI 1640 (L0495; Bio-West, Logan, UT, USA), PC-3 in F-12K (21127-022; Life Technologies, Carlsbad, CA, USA) plus 10% fetal bovine serum (FBS; S181P; Bio-West) in a humidified incubator with a 5% CO 2 atmosphere at 37°C. Then, the indicated concentrations of dihydrotestosterone (DHT), FGF-8b, and the inhibitors were added, respectively, to the culture medium which has been replaced with serum-free fresh medium.
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