Superoxide dismutase sod kit

The lipopolysaccharide (LPS) and Glutathione (GSH) assay kits were purchased from Sigma-Aldrich Chemical Company (St. Louis, USA). DAPI was from Thermo Fisher Scientific. Mouse recombinant MFG-E8 protein was from R&D Systems (Minneapolis, USA). F12-Dulbecco's modified Eagle's medium (DMEM), fetal bovine serum (FBS), phosphate-buffered saline (PBS), and penicillin/streptomycin came from Life Technologies (New York, USA). The Iba-1 antibody was from Wako (Fuji, Japan). The TRIzol reagent was purchased from Invitrogen (Waltham, USA). Kelch-like ECH-associated protein 1 (Keap-1), Nuclear factor E2-related factor 2 (Nrf-2), Heme oxygenase 1 (HO-1), and GAPDH antibodies were purchased from Cell Signaling Technology (Beverly, USA). The Malondialdehyde (MDA) kit, Superoxide Dismutase (SOD) kit, ROS assay kit, TNF-α ELISA kit, and IL-6 ELISA kit were purchased from Beyotime (Shanghai, China).

AngII was purchased from Sigma-Aldrich (St. Louis, MO, USA). Wog was purchased from Nanjing Spring & Autumn Biological Engineering (Nanjing, China) and dissolved in dimethyl sulfoxide for in vitro experiments. Anti-myosin heavy chain-beta (MyHC-β) and anti-Nrf-2 were obtained from Abcam (Cambridge, MA, USA). Anti-Keap-1, anti-Tubulin, anti-GAPDH, and secondary antibodies were purchased from Cell Signaling Technology (Danvers, MA, USA). Dihydroethidium (DHE) staining, malondialdehyde (MDA) kit, and superoxide dismutase (SOD) kit were purchased from Beyotime Institute of Biotechnology (Haimen, China). Rhodamine phalloidin was obtained from SolarBio (Beijing, China). Real-time RT-PCR reagents were purchased from TaKaRa Company (Dalian, China). All cell culture reagents were purchased from Gibco (Carlsbad, CA, USA).