10 μM thick sections were obtained from formalin-fixed and paraffin-embedded tumors and stained using an anti-phospho PKCζ Thr560 antibody (Abcam, ab62372) at a dilution of 1:100. For breast PDX and cell line-derived xenograft tumors, fresh frozen tumor pieces were mounted on Optimal Cutting Temperature (OCT) compound and sectioned to 10 μM thickness. To assess the presence of activated natural killer cells, slides were stained using goat anti-mouse NKp46/ncr1 polyclonal antibody (R and D systems, 170-6516) at a final concentration of 3 μg/ml. Images were captured on a Hamamatsu NanoZoomer, and staining was quantified using the IHC Profiler plug-in for ImageJ.
Ab62372
Manufactured by Abcam
Sourced in United States
Ab62372 is a product offered by Abcam, a leading provider of high-quality, innovative life science research tools and reagents. This product is a type of lab equipment, but no further details about its core function can be provided while maintaining an unbiased and factual approach without extrapolation.
Automatically generated - may contain errors
Lab products found in correlation
Nanozoomer, by Hamamatsu Photonics (1 mentions)
Occludin, by Thermo Fisher Scientific (1 mentions)
Ab49776, by Abcam (1 mentions)
Dynasore, by Merck Group (1 mentions)
Jam a, by Thermo Fisher Scientific (1 mentions)
Sc 17781, by Santa Cruz Biotechnology (1 mentions)
Sc 12894 r, by Santa Cruz Biotechnology (1 mentions)
2 protocols using ab62372
1
Quantifying Activated NK Cells in Tumors
2
Endothelial Cell Junction Protein Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Par3 (07-330, EMD Millipore), Par6 (ab49776 and ab6022, Abcam, Cambridge, MA, USA), aPKCζ (sc-17781, Santa Cruz Biotechnology, Dallas, TX, USA), p-aPKCζ–T560 (ab62372, Abcam, Cambridge, MA, USA), p-aPKCζ–T410 (sc-12894R, Santa Cruz Biotechnology, Dallas, TX, USA), actin (A2066, Sigma-Aldrich, St. Louis, MO, USA), F-actin BODIPY 558/568 Phalloidin (B3475, Invitrogen, Life Technologies Carlsbad, CA, USA), occludin (33-1500, Invitrogen, Life Technologies, Carlsbad, CA, USA), JAM-A S285 (sc-17430, Santa Cruz Biotechnology, Dallas, TX, USA), JAM-A Y280 (600-401-GN5, Rockland Immunochemicals Inc, Limerick, PA), JAM-A (361700, Invitrogen, Life Technologies, Carlsbad, CA, USA), and ZO-1 (61-7300, Invitrogen, Life Technologies, Carlsbad, CA, USA). Secondary antibodies used for immunofluorescence were Alexa Fluor (Life Technologies, Carlsbad, CA, USA). Dynasore (Sigma-Aldrich, St. Louis, MO, USA) was used at 80 µM. aPKCζ pseudosubstrate (539624, Millipore-Sigma, Burlington, MA, USA) was used at 5 and 10 µM.
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