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Anti mouse 1 a 1 e mhc 2 pe

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Anti-mouse I-A/I-E (MHC II)-PE is a flow cytometry reagent used to detect major histocompatibility complex (MHC) class II molecules on the surface of mouse cells. It is a monoclonal antibody conjugated to the fluorescent dye phycoerythrin (PE).

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Lab products found in correlation

Anti mouse cd86 percp, by BD (2 mentions) Anti mouse cd80 alexa fluor 647, by BD (2 mentions) Anti mouse cd40 fitc, by BD (2 mentions)

Close spelling variants of this product

MHC-II-PE Anti-I-A/I-E Anti-MHC-I Anti-MHC-II MHC-I MHC-II Mouse anti

2 protocols using anti mouse 1 a 1 e mhc 2 pe

1

Immune Cell Activation Analysis

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RAW 264.7 macrophages and DC2.4 DCs were respectively seeded in 12-well plates and incubated overnight for experiment. Svg3 was transfected using Lipofectamine reagent at a final concentration of 25 nM. After treatment for 24 h, the cells were collected and stained with anti-mouse CD86-PerCp, anti-mouse CD80-Alexa Fluor 647, anti-mouse CD40-FITC, or anti-mouse I-A/I-E (MHC II)-PE, respectively, for flow cytometry (BD Canto).
Zhou S., Su T., Cheng F., Cole J., Liu X., Zhang B., Alam S., Liu J, & Zhu G. (2024). Engineering cGAS-agonistic oligonucleotides as therapeutics for cancer immunotherapy. Molecular Therapy. Nucleic Acids, 35(1), 102126.
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2

Macrophage and DC Activation Assay

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RAW 264.7 macrophages and DC2.4 DCs were respectively seeded in 12-well plate and incubated overnight for experiment. Svg3 was transfected using lipofectamine reagent at a final concentration of 25 nM. After treatment for 24 h, the cells were collected and stained with anti-mouse CD86-PerCp, anti-mouse CD80-Alexa Fluor 647, anti-mouse CD40-FITC, anti-mouse I-A/I-E (MHC II)-PE respectively for flow cytometry (BD Canto).
Zhou S., Su T., Cheng F., Cole J., Liu X., Zhang B., Alam S., Liu J, & Zhu G. (2023). Engineering cGAS-agonistic oligonucleotides as therapeutics and vaccine adjuvants for cancer immunotherapy. bioRxiv.
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