Vitamin e α tocopherol acetate

Immediately after plating in 96-well plates, the SOSR-COs were treated with various drugs for 48 h. Along with VPA (Cayman Chemical), the SOSR-COs were treated with different HDAC inhibitors, GSK3β inhibitors, folic acid (FA) inhibition or supplementation, and antioxidants. We tested HDAC inhibitors that target different classes: Trichostatin A (Cayman Chemical), Nicotinamide (Cayman Chemical), and CI-994 (Cayman Chemical). The GSK3β inhibitors included CHIR99021 (Cayman Chemical), BIO (Cayman Chemical), SB-216763 (Cayman Chemical), SB-415286 (Cayman Chemical), and lithium chloride (Sigma-Aldrich, St. Louis, MO, USA). FA (Cayman Chemical) was supplemented, and the FA pathway was inhibited by aminopterin (Cayman Chemical). The antioxidant was vitamin E (±-α-Tocopherol Acetate; Cayman Chemical). Appropriate amounts of each treatment were mixed with 3N media without vitamin A, plus the 4 inhibitors. A total of 100 μL of media mixed with the treatment was added per well.

Immediately after plating in 96-well plates, SOSRS were treated with various drugs for 48 hours. Along with VPA (Cayman Chemical, 13033), SOSRS were treated with different HDAC inhibitors, GSK3β inhibitors, folic acid (FA) inhibition or supplementation, and antioxidants. We tested HDAC inhibitors that target different classes: Trichostatin A (TSA; Cayman Chemical, 89730), Nicotinamide (Cayman Chemical, 11127), CI-994 (Cayman Chemical, 12084). GSK3β inhibitors included CHIR99021 (Cayman Chemical, 13122), Bio (Cayman Chemical, 13123), SB-216763 (Cayman Chemical, 10010246), SB-415286 (Cayman Chemical, 10010247), and lithium chloride. FA (Cayman Chemical, 20515) was supplemented, and the FA pathway was inhibited by Aminopterin (Cayman Chemical, 21802). Antioxidants included Vitamin E (±-α-Tocopherol Acetate; Cayman Chemical, 28399), and Resveratrol (trans-Resveratrol [RV]; Cayman Chemical, 70675). Appropriate amounts of each treatment were mixed with 3N media without vitamin A plus the 4 inhibitors. 100μL of media mixed with the treatment was added per well. SOSRS were treated for 48 hours before being fixed.