Rat kidneys were collected, fixed with 4% paraformaldehyde, embedded in paraffin, and sectioned 5 μm thickness) for the evaluation of crystal deposition using von Kossa staining (Solarbio, Beijing, China). Paraffinembedded tissues were sectioned into 3-μm-thick sections for immunohistochemical staining. Sections were dewaxed, dehydrated, and soaked into 0.01 mol/L sodium citrate to repair antigen using the high pressure method. Then 3% hydrogen peroxide was used to remove the endogenous peroxide. Sections were blocked by goat serum albumin and incubated with the primary antibody overnight at 4 C. Following the incubation with the secondary antibody for 30 min, sections were stained with 3,3-diaminoaniline and counterstained with hematoxylin for 1 min, and then sealed with neutral resin. multiple groups was performed using analysis of variance. p < .05 was considered statistically significant. # p < .05, ## p < .01, ### p < .001 vs. control group, * p < .05, ** p < .01, *** p < .001 vs. model group.
Von kossa staining
Manufactured by Solarbio
Sourced in China
Von Kossa staining is a histochemical technique used to detect the presence of calcium deposits in tissue samples. It involves the treatment of the sample with silver nitrate, which reacts with the calcium to form silver phosphate or silver carbonate. The stained sample is then exposed to light, causing the silver compounds to be reduced to metallic silver, which appears as a dark brown or black stain. This staining procedure is commonly used in the analysis of mineralized tissues, such as bone and cartilage.
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Lab products found in correlation
2 protocols using von kossa staining
1
Rat Kidney Crystal Deposition Analysis
2
Apoptosis and Crystal Deposition in Kidneys
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Apoptoses of the kidney tissues were assessed with TUNEL assay, following the manufacturer’s instructions (Vazyme). Images were captured using a BX53 fluorescence microscope (Olympus, Tokyo, Japan). The crystal depositions in the kidneys were analyzed by Von Kossa staining according to manufacturer’s instructions (Solarbio, Beijing). The stained tissues were observed by microscopy (Olympus, Japan).
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