The largest database of trusted experimental protocols

2 protocols using cd152 apc

1

Dioscin Modulates Regulatory T Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
Dioscin (purity ≥ 98%; lot number: B21176) was purchased from Shanghai Yanye Bio-Technology Co., Ltd (Shanghai, China). Mouse IL-10, IL-35 and TGF-β ELISA KIT were purchased from Sangon Biotech (Shanghai, China). CD4 + CD25+ Regulatory T Cell Isolation Kit was purchased from Miltenyi Biotec (Bergisch Gladbach, Germany). The fluorophore-conjugated monoclonal antibodies: CD4-FITC, CD25-PE, Foxp3-APC, CD127-APC, CD152-APC, CD357-PerCP, CD39-PerCP, CD73-FITC and Perforin-APC were from Biolegend (San Diego, CA, USA).
+ Open protocol
+ Expand
2

Characterization of Lymph Node Immune Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
Ipsilateral draining submandibular and cervical lymph nodes were extracted from recipient mice (n = 6) of each group at the time of euthanization. The single-cell suspension from the lymph nodes was centrifuged and the final cell concentration was adjusted to 1 × 10 7 /mL. The 100-µl cell suspension was taken to the bottom of the flow tube. Surface staining was performed before intracellular staining. CD4-APC/C7 (Biolegend, clone # GK1.5), CD44-BV510 (Biolegend, clone # IM7), CD62-FITC (Biolegend, clone #MEL-14), CD152-APC (Biolegend, clone #UC10-4B9), GITR-PE/Cy7 (Biolegend, clone #DTA-1), Nrp-1-BV421 (Biolegend, clone #3E12), CD25-APC (Biolegend, clone #PC61), CD62L-FITC (Biolegend, clone #MEL-14), TIGIT-BV421 (Biolegend, clone #1G9), CD226-PE/Cy7 (Biolegend, clone #10E5), Helios-PerCP/Cy5.5 (Biolegend, clone #22F6), and Foxp3-PE (Thermo, clone# FJK-16s) were used in this study. Fluorescence-activated cell sorting (FACS) was performed using BD FACS LSRII Flow Cytometer. Fifty thousand lymphocytes for each sample were collected by FACS Diva 8.0 software and finally analyzed by Flowjo software (BD Bioscience, USA).
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!