Stool samples were plated on eosin methylene blue agar (Liofilchem, Italy) and the plates incubated at +37 °C for 18–24 h. After incubation, the suspected E. coli colonies (dark purple colonies with a metallic sheen) were selected and streaked onto Mueller Hinton agar plate (Liofilchem, Italy). Confirmation was carried out by a biochemical microbiology method based on negative urease (Bio-Rad, France), negative citrate (Liofilchem, Italy), positive indole (Bio-Rad, France), positive lactose (Lioflchem, Italy), and positive orthonitrophenyl-β-D-galactopyranoside (ONPG) (bioMerieux, France). E. coli strains isolated were confirmed by API 20E (bioMérieux, France). The 16-plex polymerase chain reaction (PCR) was used to characterize the five main pathogroups of E. coli as described by Antikainen et al. [9 (link)].
Indole
Manufactured by Bio-Rad
Sourced in France
Indole is a chemical compound commonly used in biochemical and molecular biology laboratories. It serves as a core component in various analytical and diagnostic procedures. The primary function of Indole is to facilitate specific chemical reactions and detection methods, enabling researchers to analyze and identify biomolecules in their samples.
Automatically generated - may contain errors
3 protocols using indole
1
Identification and Characterization of E. coli
2
Isolation and Identification of E. coli Pathogroups
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Stool samples were plated on eosin methylene blue agar (Liofilchem, Italy), and the plates were incubated at +37°C for 18-24 h. After incubation, the suspected colonies were selected and streaked onto Mueller Hinton agar plate (Liofilchem, Italy). Confirmation was carried out by a biochemical microbiology method based on negative urease (Bio-Rad, France), negative citrate (Liofilchem, Italy), positive indole (Bio-Rad, France), positive lactose (Liofi lchem, Italy), and positive orthonitrophenyl-β-Dgalactopyranoside (ONPG) (bioMerieux, France). E. coli strains isolated were confirmed by API 20E (bioMérieux, France).
The 16-plex PCR was used to detect simultaneously 16 genes from the five main pathogroups of E. coli (enterohemoragic E. coli: EHEC, enteropathogenic E. coli: EPEC, enteroaggregative E. coli: EAEC, enteroinvasive E. coli: EIEC and enterotoxigenic E. coli: ETEC) as described by Antikainen et al. 15 . The genes investigated and primers used are listed in Table 1 [15] [16] [17] .
The 16-plex PCR was used to detect simultaneously 16 genes from the five main pathogroups of E. coli (enterohemoragic E. coli: EHEC, enteropathogenic E. coli: EPEC, enteroaggregative E. coli: EAEC, enteroinvasive E. coli: EIEC and enterotoxigenic E. coli: ETEC) as described by Antikainen et al. 15 . The genes investigated and primers used are listed in Table 1 [15] [16] [17] .
3
Detection of Pathogenic E. coli using 16-plex PCR
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Stool samples were plated on eosin methylene blue agar (Lio lchem, Italy), and the plates were incubated at + 37 °C for 18-24 h. After incubation, the suspected colonies were selected and streaked onto Mueller Hinton agar plate (Lio lchem, Italy). Con rmation was carried out by a biochemical microbiology method based on negative urease (Bio-Rad, France), negative citrate (Lio lchem, Italy), positive indole (Bio-Rad, France), positive lactose (Lio lchem, Italy), and positive orthonitrophenyl-β-D-galactopyranoside (ONPG) (bioMerieux, France). E. coli strains isolated were con rmed by API 20E (bioMérieux, France).
The 16-plex PCR was used to detect simultaneously 16 genes from the ve main pathogroups of E. coli (enterohemoragic E. coli: EHEC, enteropathogenic E. coli: EPEC, enteroaggregative E. coli: EAEC, enteroinvasive E. coli: EIEC and enterotoxigenic E. coli: ETEC) as described by Antikainen et al. [15] (link). The genes investigated and primers used are listed in Table 1 [15] (link)[16] [17] (link).
The 16-plex PCR was used to detect simultaneously 16 genes from the ve main pathogroups of E. coli (enterohemoragic E. coli: EHEC, enteropathogenic E. coli: EPEC, enteroaggregative E. coli: EAEC, enteroinvasive E. coli: EIEC and enterotoxigenic E. coli: ETEC) as described by Antikainen et al. [15] (link). The genes investigated and primers used are listed in Table 1 [15] (link)[16] [17] (link).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!