Hct116 cl 0096

Human CRC HCT116 (CL-0096), human SW480 (CL-0223A) and mouse CT-26 (CL-0071) cells were purchased from Procell and cultured in an incubator with 5% CO₂ and at a temperature of 37 °C. The composition of the culture medium consisted of DMEM or RPMI-1640/newborn bovine serum/double antibody (penicillin + streptomycin) = 10:1:1.

Human CRC cell line HCT116 (CL-0096) was obtained from Procell Life Science & Technology (China), while the NCM460 (iCell-h373) cell line, representing normal colonic epithelial cells, was acquired from Cellverse (China). HCT116 cells were propagated in DMEM (C11995500BT, Gibco, USA), and NCM460 cells were cultivated in RPMI 1640 medium (10-040-CV, Corning, USA). Both media were fortified with 10% fetal bovine serum (FBS; C0235, Gibco, USA) and a combination of 100 U/ml penicillin and 100 µg/ml streptomycin (C0222, Beyotime, China). Cell cultures were incubated at 37 °C in a humidified incubator with a 5% CO2 atmosphere.