Rabbit anti mag

Rabbit anti-ERLI (UM510) and Rabbit anti-CLPI (UM377, 1:250–500) were a kind gift from Dr Margolis, University of Michigan, USA).
Rabbit anti-pan-Crb3 (C2, kind gift from Dr A.Le Bivic, IBDML, France, 1:250)
Rabbit anti-YAP (#4912, Cell Signaling, 1:1,000)
Rabbit anti-phospho-YAP (S127A) (#4911, Cell Signaling, 1:1,000)
Rabbit anti-GFP (A11122, Invitrogen, 1:500)
Mouse anti-FLAG (F-3165, Sigma, 1:500)
Rabbit anti-MAG (#9086, Cell Signaling, 1:1,000)
Goat anti-MPZ (PA5-18773, Thermo Fisher, 1:3,000)
Rabbit anti-Rab11a (#2413, Cell Signaling, 1:1,000)
Rabbit anti-lamininγ1 (NBP1-19643, Novus Biologicals, 1:500)
Mouse anti-MBP (SMI94, Sternberger Monoclonal incorporated, 1:1,000)
Mouse anti-α-β-Actin (C1.AC-15, #A1978, Sigma Aldrich, 1:10,000)
Rabbit Pan-TEAD (D3F7L, Cell Signaling, 1:1,000)
Mouse anti-AKT (Cell signaling, 1:1,000)
Mouse anti-P-AKT (ser473, 587F11, Cell signaling, 1:1,000)
Secondary antibodies, Peroxidase Goat anti Rabbit or Mouse (H+L) (Jackson Immuno Research) were used at 1:10,000 dilution.

Cells were fixed with 4% paraformaldehyde (PFA; Merck) at room temperature for 30 min and permeabilized with PBS containing 0.1% Triton X-100 for 15 min. Then, cells were blocked with 3% normal donkey serum (Sigma-Aldrich) for 1 h at room temperature and treated with primary antibody overnight at 4 °C. Primary antibodies were used as follows; rabbit anti-βIII-Tubulin (1:2000, PRB-435P; Biolegend), rat anti-MBP (1:500, ab7349; Abcam), rabbit anti-MAG (1:500, 9043; Cell Signaling Technology), mouse anti-GFAP (1:1000, GA-5, Sigma Aldrich), rat anti-GFAP (1:500, 2.2B10; Thermo Fisher Scientific), goat anti-PDGFRα (1:1000, AF1062; R&D Systems), rabbit anti-Ki67 (1:500, ab16667; Abcam), mouse anti-Olig2 (1:1000, 211F1.1; Millipore), rat anti-Nestin (1:1000, rat-401; Millipore). After three times wash, cells were incubated with secondary antibodies at room temperature for 1 h. Secondary antibodies were used as follows, Alexa 488-conjugated donkey anti-mouse IgG, Alexa 594-conjugated donkey anti-rat IgG (1:500; Thermo Fisher Scientific) and DAPI (1:2000; DOJINDO). After three times wash, cells were observed with IN Cell Analyzer 2000 (GE Healthcare) or confocal laser scanning microscope (FV3000, Olympus).