Transwell assay was used to investigate the migration and invasion of GC cells. Different group GC cells were put on the upper Matrigel-coated invasion chambers or non-coated migration chambers (BD Biosciences, USA), respectively. 500 μl of DMEM medium containing 10% FBS was put in the lower chamber, and the serum-free medium was put in the upper chamber. The non-invasive cells were wiped off by cotton swabs after 48 hours of incubation. The migrating and invading cells were xed with 95% ethanol, stained with 0.1% crystal violet. The number of migratory and invasive cells in the lower chamber were counted under an inverted microscope (×100). The experiments were independently repeated three times.
Non coated migration chambers
Manufactured by BD
Sourced in United States
Non-coated migration chambers are a type of lab equipment used to study cell migration. They provide a platform for observing and analyzing the movement of cells through a porous membrane under controlled conditions. The core function of these chambers is to facilitate the study of cell migration without the influence of additional coatings or treatments.
Automatically generated - may contain errors
Lab products found in correlation
4 protocols using non coated migration chambers
1
Transwell Assay for GC Cell Migration and Invasion
2
Transwell Assay for GC Cell Migration and Invasion
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Transwell assay was used to investigate the migration and invasion of GC cells. Different group GC cells were put on the upper Matrigel-coated invasion chambers or non-coated migration chambers (BD Biosciences, USA), respectively. 500 μl of DMEM medium containing 10% FBS was put in the lower chamber, and the serum-free medium was put in the upper chamber. The non-invasive cells were wiped off by cotton swabs after 48 hours of incubation. The migrating and invading cells were fixed with 95% ethanol, stained with 0.1% crystal violet. The number of migratory and invasive cells in the lower chamber were counted under an inverted microscope (×100).
The experiments were independently repeated three times.
The experiments were independently repeated three times.
3
Transwell Assay for GC Cell Migration and Invasion
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Transwell assay was used to investigate the migration and invasion of GC cells. Different group GC cells were put on the upper Matrigel-coated invasion chambers or non-coated migration chambers (BD Biosciences, USA), respectively. 500 μl of DMEM medium containing 10% FBS was put in the lower chamber, and the serum-free medium was put in the upper chamber. The non-invasive cells were wiped off by cotton swabs after 48 hours of incubation. The migrating and invading cells were xed with 95% ethanol, stained with 0.1% crystal violet. The number of migratory and invasive cells in the lower chamber were counted under an inverted microscope (×100). The experiments were independently repeated three times.
4
Transwell Assay for GC Cell Migration and Invasion
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Transwell assay was used to investigate the migration and invasion of GC cells. Different group GC cells were put on the upper Matrigel-coated invasion chambers or non-coated migration chambers (BD Biosciences, USA), respectively. 500 μl of DMEM medium containing 10% FBS was put in the lower chamber, and serum-free medium was put in the upper chamber. The non-invasive cells were wiped off by cotton swabs after 48 hours of incubation. The migrating and invading cells were xed with 95% ethanol, stained with 0.1% crystal violet. The number of migratory and invasive cells in the lower chamber were counted under an inverted microscope (×100). The experiments were independently repeated for three times.
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