Use cold phosphate-buffered saline (PBS) wash the cells and suspend 2× 10 5 in 100µl of 1× binding buffer. Then, use Annexin PE/7-AAD Apoptosis Detection Kit (Vazyme, China) according to the manufacturer's instructions. For cell cycle assay, 1 × 10 6 cells were washed and re-suspended in cold PBS and immobilized in 70% ethanol overnight at 4℃. Apoptosis and cell cycle were detected by Coulter FC500 ow cytometry (Beckman, USA).
Coulter fc500 ow cytometry
Manufactured by Beckman Coulter
Sourced in United States
The Coulter FC500 flow cytometry system is a versatile and reliable instrument designed for advanced cell analysis. It utilizes flow cytometry technology to rapidly measure and analyze the physical and fluorescent characteristics of cells or particles suspended in a fluid stream. The Coulter FC500 provides accurate and reproducible data, enabling researchers and clinicians to obtain valuable insights into cell populations and their behaviors.
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Lab products found in correlation
2 protocols using coulter fc500 ow cytometry
1
Apoptosis and Cell Cycle Analysis
2
Detecting DNA Repair Mechanisms
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Direct-repeat (DR)-GFP Plasmid pDRGFP(Addgene, USA) was used for detecting HR ligation and pimEJ5GFP(Addgene, USA) was used for detecting NHEJ ligation. Cells were transfected by lipo8000 Reagent (Beyotime, China). Subsequently transfected pCBASce-plasmid (Addgene, USA) in 48h. GFP signal was detected in 48h post-transfection on Coulter FC500 ow cytometry (Beckman, USA).
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