Herdchek csfv ab elisa kit

The yeast expression vector pPIC9K and the yeast P. pastoris GS115 strain were purchased from Invitrogen (Carlsbad, USA).
The following serum samples were tested in this study: (1) 254 swine serum samples confirmed to be free of CSFV antibodies using the virus neutralization test (VNT) and the HerdChek CSFV Ab ELISA kit (IDEXX, USA) for cutoff value determination; (2) sequential serum samples from 9 pigs upon vaccination with CSFV C-strain or marker vaccine rAdV-SFV-E2, and subsequent challenge with CSFV virulent Shimen strain; (3) a panel of swine sera for comparison of yE rns -iELISA with VNT, including 63 sera from pigs experimentally infected with the CSFV Shimen strain, 18 C-strain-vaccinated swine sera, 34 rAdV-SFV-E2-immunized swine sera and 101 clinical swine serum samples.
Other serum samples included 3 sera from pigs experimentally infected with porcine reproductive and respiratory syndrome virus (PRRSV), 3 sera from pigs experimentally infected with pseudorabies virus (PRV), 3 sera from pigs infected with porcine circovirus type 2 (PCV2), 3 sera from pigs experimentally infected with porcine epidemic diarrhea virus (PEDV), 19 sera from pigs experimentally infected with BVDV (4 sera from the EU Reference Laboratory for CSF, Hannover, Germany) and 4 sera from pigs experimentally infected with BDV (from the EU Reference Laboratory for CSF).

The specificity of the assay was tested by using 63 sera of pigs experimentally infected with CSFV and 254 sera of non-infected pigs, as confirmed using VNT and the HerdChek CSFV Ab ELISA kit (IDEXX, USA). In addition, the sera of pigs experimentally infected with PRRSV, PRV, BVDV, BDV, PCV2 or PEDV were used to evaluate the cross-reactivity with the assay.
A panel of swine sera was tested in parallel with both yE rns -iELISA and VNT, including 63 sera from pigs experimentally infected with CSFV Shimen strain, 18 C-strain-vaccinated swine sera, 34 rAdV-SFV-E2-immunized swine sera and 101 clinical swine serum samples. VNT was performed as described previously (Li et al., 2009) .