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Liss rhodamine

Manufactured by Avanti Polar Lipids
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LISS-Rhodamine is a fluorescent probe used for labeling and tracking lipids in biological systems. It consists of a rhodamine dye conjugated to the lipid LISS (Lipid Intercalating Sulfo-rhodamine). This product provides a convenient way to label and visualize lipids in cells and tissues.

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Lab products found in correlation

200 nm ptfe filter, by Merck Group (2 mentions) Biotinyl cap pe, by Avanti Polar Lipids (2 mentions) Dgs nta ni, by Avanti Polar Lipids (2 mentions)

2 protocols using liss rhodamine

1

Preparation of Biotinylated and Fluorescent SUVs

Check if the same lab product or an alternative is used in the 5 most similar protocols
SUVs were prepared by rehydrating a lipid film composed primarily of POPC (1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine, Avanti Polar Lipids), doped with 0.5% of Biotinyl Cap PE (1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-(cap biotinyl), Avanti Polar Lipids), 1% DGS-Ni-NTA (1,2-dioleoyl-sn-glycero-3-[(N-(5-amino1-carboxypentyl)iminodiacetic acid)succinyl] with nickel salt, Avanti Polar Lipids), and 0.4% LISS-Rhodamine (1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-(lissamine rhodamine B sulfonyl), Avanti Polar Lipids) in pure deionized H2O. The rehydrated solution was vortexed briefly and then sonicated using a tip-sonicator at 20% power pulsing off and on for 3 minutes. SUVs were then filtered through a 200 nm PTFE filter (Millipore). SUV solutions were stored at 4°C and used within 48 hours of preparation to avoid phospholipid oxidization.
Suter E.C., Schmid E.M., Harris A.R., Voets E., Francica B, & Fletcher D.A. (2021). Antibody:CD47 ratio regulates macrophage phagocytosis through competitive receptor phosphorylation. Cell reports, 36(8), 109587.
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2

Preparation of Biotinylated and Fluorescent SUVs

Check if the same lab product or an alternative is used in the 5 most similar protocols
SUVs were prepared by rehydrating a lipid film composed primarily of POPC (1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine, Avanti Polar Lipids), doped with 0.5% of Biotinyl Cap PE (1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-(cap biotinyl), Avanti Polar Lipids), 1% DGS-Ni-NTA (1,2-dioleoyl-sn-glycero-3-[(N-(5-amino1-carboxypentyl)iminodiacetic acid)succinyl] with nickel salt, Avanti Polar Lipids), and 0.4% LISS-Rhodamine (1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-(lissamine rhodamine B sulfonyl), Avanti Polar Lipids) in pure deionized H2O. The rehydrated solution was vortexed briefly and then sonicated using a tip-sonicator at 20% power pulsing off and on for 3 minutes. SUVs were then filtered through a 200 nm PTFE filter (Millipore). SUV solutions were stored at 4°C and used within 48 hours of preparation to avoid phospholipid oxidization.
Suter E.C., Schmid E.M., Harris A.R., Voets E., Francica B, & Fletcher D.A. (2021). Antibody:CD47 ratio regulates macrophage phagocytosis through competitive receptor phosphorylation. Cell reports, 36(8), 109587.
+ Open protocol
+ Expand

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