Log In Sign up
The largest database of trusted experimental protocols

Gdc 0941

Manufactured by LC Laboratories
Visit Supplier
Sourced in United States

GDC-0941 is a potent and selective inhibitor of phosphoinositide-3-kinase (PI3K). It inhibits the catalytic activity of the p110α, p110β, p110δ, and p110γ isoforms of PI3K. GDC-0941 is a small molecule compound used for research purposes.

Automatically generated - may contain errors

Lab products found in correlation

Lapatinib, by LC Laboratories (5 mentions) Mda mb 231, by American Type Culture Collection (3 mentions) Mcf 7, by American Type Culture Collection (3 mentions) Carboxyfluoreszein succinimidyl ester cfse, by Thermo Fisher Scientific (3 mentions) Saracatinib, by LC Laboratories (3 mentions) Bt474, by American Type Culture Collection (2 mentions) Hcc38, by American Type Culture Collection (2 mentions) Hcc1428, by American Type Culture Collection (2 mentions) Mda mb 453, by American Type Culture Collection (2 mentions) Bt 20, by American Type Culture Collection (2 mentions) Pd0325901, by LC Laboratories (2 mentions) Mk2206, by LC Laboratories (2 mentions) Mln8237, by LC Laboratories (2 mentions) Lee011, by LC Laboratories (2 mentions) Sum52pe, by American Type Culture Collection (2 mentions) Mk 2206, by Selleck Chemicals (2 mentions) Herceptin, by BOC Sciences (2 mentions) Doxycycline, by Merck Group (2 mentions) Vincristine, by Merck Group (2 mentions) Rapamycin, by LC Laboratories (2 mentions)

Spelling variants of this product

GDC-0941 free base (2 citations)

21 protocols using gdc 0941

1

Breast Cancer Cell Line Characterization

Check if the same lab product or an alternative is used in the 5 most similar protocols
BT549 and SKBR3 cells were obtained from the UCSF Cell Culture Facility.
BT20, BT474, HCC1428, HCC38, LY2, MCF7, MDAMB231, MDAMB453, T47D, SUM52PE, and
ZR75B cell lines were obtained from the American Type Culture Collection (ATCC).
Cell lines used for proteomic profiling and molecular analyses were
authenticated by STR analysis. Lines were grown according to published
protocols50 except for
SKBR3 which was cultured using RPMI media supplemented with 10% fetal
bovine serum (FBS) and 1% pen/strep. All cell lines tested negative for
mycoplasma contamination. Drugs used for cell culture experiments in this study
were purchased from Selleck Chemicals (GDC-0941, MK2206, PD0325901, Lapatinib,
MLN8237, and LEE011) and LC Laboratories (RAD001).
Donnella H.J., Webber J.T., Levin R.S., Camarda R., Momcilovic O., Bayani N., Shah K.N., Korkola J., Shokat K.M., Goga A., Gordan J.D, & Bandyopadhyay S. (2018). Kinome rewiring reveals AURKA limits PI3K-pathway inhibitor efficacy in breast cancer. Nature chemical biology, 14(8), 768-777.
+ Open protocol
+ Expand
2

Insulin Stimulation and Pathway Inhibition

Check if the same lab product or an alternative is used in the 5 most similar protocols
For insulin stimulation, cells were serum-starved for 20–24 h and stimulated with 100 nM insulin (Life Technologies). Inhibitors were used as follows: GDC-0941 (LC Laboratories), 1 μM; GDC-0068 (Active Biochem), 1 μM; MK2206 (Selleck Chemicals), 1 μM; rapamycin (Cayman Chemicals), 100 nM; Torin1 (Tocris), 1 μM; sulfasalazine (SSA) (Sigma), 1 mM.
Lien E.C., Ghisolfi L., Geck R.C., Asara J.M, & Toker A. (2017). Oncogenic PI3K Promotes Methionine Dependency in Breast Cancer Cells Through the Cystine-Glutamate Antiporter xCT. Science signaling, 10(510), eaao6604.
+ Open protocol
+ Expand
3

Breast Cancer Cell Line Characterization

Check if the same lab product or an alternative is used in the 5 most similar protocols
BT549 and SKBR3 cells were obtained from the UCSF Cell Culture Facility.
BT20, BT474, HCC1428, HCC38, LY2, MCF7, MDAMB231, MDAMB453, T47D, SUM52PE, and
ZR75B cell lines were obtained from the American Type Culture Collection (ATCC).
Cell lines used for proteomic profiling and molecular analyses were
authenticated by STR analysis. Lines were grown according to published
protocols50 except for
SKBR3 which was cultured using RPMI media supplemented with 10% fetal
bovine serum (FBS) and 1% pen/strep. All cell lines tested negative for
mycoplasma contamination. Drugs used for cell culture experiments in this study
were purchased from Selleck Chemicals (GDC-0941, MK2206, PD0325901, Lapatinib,
MLN8237, and LEE011) and LC Laboratories (RAD001).
Donnella H.J., Webber J.T., Levin R.S., Camarda R., Momcilovic O., Bayani N., Shah K.N., Korkola J., Shokat K.M., Goga A., Gordan J.D, & Bandyopadhyay S. (2018). Kinome rewiring reveals AURKA limits PI3K-pathway inhibitor efficacy in breast cancer. Nature chemical biology, 14(8), 768-777.
+ Open protocol
+ Expand
4

Evaluation of Cancer Inhibitors

Check if the same lab product or an alternative is used in the 5 most similar protocols
Inhibitors GDC-0941(LC Laboratories, G-9252), Lapatinib (LC Laboratories, L-4899), and Herceptin (BOC Sciences, 180288-69-1) were dissolved in DMSO. DMSO was used as the control. Details are in the Supplemental Information.
Liu J., Cho S.N., Akkanti B., Jin N., Mao J., Long W., Chen T., Zhang Y., Tang X., Wistub I.I., Creighton C.J., Kheradmand F, & DeMayo F.J. (2015). ErbB2 Pathway Activation upon Smad4 Loss Promotes Lung Tumor Growth and Metastasis. Cell reports, 10(9), 1599-1613.
+ Open protocol
+ Expand
5

Pharmacological Inhibitor Acquisition Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols
We obtained rapamycin, MLN0128, GDC-0941, and NVP-BEZ235 from LC Laboratories (Woburn, MA, USA); ABT-263, ABT-199, MK2206 and GDC-0980 from Active Biochem (Wan Chai, Hong Kong), and AKT inhibitor VIII from Chemdea (Ridgewood, NJ, USA). InSolution Q-VD-OPh was obtained from EMD Millipore (Billerica, MA, USA), vincristine was obtained from Sigma-Aldrich (St. Louis, MO), dimethyl sulfoxide (DMSO) from Fisher Scientific (Waltham, MA, USA) and doxycycline from Sigma-Aldrich (St. Louis, MO).
Lee J.S., Tang S.S., Ortiz V., Vo T.T, & Fruman D.A. (2015). MCL-1-independent mechanisms of synergy between dual PI3K/mTOR and BCL-2 inhibition in diffuse large B cell lymphoma. Oncotarget, 6(34), 35202-35217.
+ Open protocol
+ Expand
6

Analyzing Cellular Signaling Pathways

Check if the same lab product or an alternative is used in the 5 most similar protocols
GDC-0941 was obtained from LC Laboratories, while etoposide (VP16) and Hoechst were provided by Sigma. Primary antibodies - pSTAT3 (Tyr705, D3A7), STAT3 (124H6), pAKT (Thr308, 244F9), pAKT (Ser473), AKT (11E7) and GAPDH were purchased from Cell Signalling while secondary antibodies were purchased from Dianova. Carboxyfluoreszein-Succinimidyl-Ester (CFSE) was obtained from Invitrogen, D-Luciferin sodium salt was purchased from PJK.
Ehrhardt M., Craveiro R.B., Holst M.I., Pietsch T, & Dilloo D. (2014). The PI3K inhibitor GDC-0941 displays promising in vitro and in vivo efficacy for targeted medulloblastoma therapy. Oncotarget, 6(2), 802-813.
+ Open protocol
+ Expand
7

Pharmacological Inhibition of PI3K Isoforms

Check if the same lab product or an alternative is used in the 5 most similar protocols
C57BL/6 mice were bred at the University of California, Irvine, and used at between 6 and 12 weeks of age. All animals were studied in compliance with protocols approved by the Institutional Animal Care and Use Committee of the University of California, Irvine. The p110δ-selective PI3K inhibitor IPI-3063 and p110δ/γ PI3K inhibitor IPI-443 were synthesized at Infinity Pharmaceuticals. These compounds and the pan-PI3K class I inhibitor GDC-0941 (LC laboratories) were dissolved in DMSO. The p110γ PI3K inhibitor AS-252424 (Chemdea) was dissolved in ethanol. Inhibitors were included throughout the indicated cell treatment periods.
Chiu H., Mallya S., Nguyen P., Mai A., Jackson L.V., Winkler D.G., DiNitto J.P., Brophy E.E., McGovern K., Kutok J.L, & Fruman D.A. (2017). The Selective Phosphoinoside-3-Kinase p110δ Inhibitor IPI-3063 Potently Suppresses B Cell Survival, Proliferation, and Differentiation. Frontiers in Immunology, 8, 747.
+ Open protocol
+ Expand
8

Evaluating Novel Epigenetic Modulators

Check if the same lab product or an alternative is used in the 5 most similar protocols
FK‐A11 was produced by Hamari Chemicals (Osaka, Japan). FK228 and FK‐A3 were provided by Tohoku Medical and Pharmaceutical University (Sendai, Japan).18 GDC‐0941 was purchased from LC Laboratories (Woburn, MA, USA). Suberoylanilidehydroxamic acid (SAHA) was purchased from Cayman Chemical Company (Ann Arbor, MA, USA). Reagents were dissolved in DMSO for the in vitro assay and in saline with 1.3% DMSO and 20% 4‐hydroxypropyl β‐cyclodextrin (ChemScene, Monmouth Junction, NJ, USA) for the in vivo experiments.
Saijo K., Imai H., Chikamatsu S., Narita K., Katoh T, & Ishioka C. (2017). Antitumor activity and pharmacologic characterization of the depsipeptide analog as a novel histone deacetylase/ phosphatidylinositol 3‐kinase dual inhibitor. Cancer Science, 108(7), 1469-1475.
+ Open protocol
+ Expand
9

Investigating STAT3 and AKT Signaling

Check if the same lab product or an alternative is used in the 5 most similar protocols
Vandetanib and GDC-0941 were obtained from LC Laboratories. The primary antibody pSTAT3 (TYR705, D3A7), STAT3 (124H6), pAKT (Ser473), AKT (11E7) and GAPDH (D18H11) were purchased from Cell Signaling while secondary antibodies were purchased from Dianova. Carboxyfluoreszein-Succinimidyl Ester (CFSE) was purchased from Invitrogen, while Hoechst 33258 was provided by Sigma.
Craveiro R.B., Ehrhardt M., Velz J., Olschewski M., Goetz B., Pietsch T, & Dilloo D. (2017). The anti-neoplastic activity of Vandetanib against high-risk medulloblastoma variants is profoundly enhanced by additional PI3K inhibition. Oncotarget, 8(29), 46915-46927.
+ Open protocol
+ Expand
10

Pharmacological Inhibitors in Cancer Research

Check if the same lab product or an alternative is used in the 5 most similar protocols
Dasatinib, GDC-0941, AZD8055, rapamycin and MLN0128 were obtained from LC Laboratories (Woburn, MA); AKT inhibitor VIII from Chemdea (Ridgewood, NJ). InSolution Q-VD-OPh was from EMD Millipore (Billerica, MA); vincristine, doxycycline, etoposide, daunorubicin, MTX, 6-MP, dexamethasone and araC are from Sigma-Aldrich (St. Louis, MO). Palbociclib was purchased from Selleck Chemicals (Houston, TX).
Vo T.T., Lee J.H., Nguyen D., Lui B., Pandori W., Khaw A., Mallya S., Lu M., Müschen M., Konopleva M, & Fruman D.A. (2017). mTORC1 Inhibition Induces Resistance to Methotrexate and 6-Mercaptopurine in Ph+ and Ph-like B-ALL. Molecular cancer therapeutics, 16(9), 1942-1953.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!