As previously described,37 (link) intact fetuses (ED 18.5) were weighed, crown-rump length measured, and then reduced to ash in a furnace (500 °C × 24 hr). A Perkin Elmer 2380 Atomic Absorption Flame Spectrophotometer assayed the calcium and magnesium content of the ash, while the phosphorus content was determined using a colorimetric assay (Sekisui Diagnostics).
Colorimetric assay
Manufactured by Sekisui
The Colorimetric Assay is a laboratory equipment used to quantify the concentration of a specific substance in a solution. It measures the absorbance or color intensity of the solution, which is directly proportional to the concentration of the target analyte. This equipment is commonly used in various scientific and analytical applications, such as biochemistry, cell biology, and environmental testing, to determine the levels of various compounds, proteins, or other biomolecules.
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Lab products found in correlation
3 protocols using colorimetric assay
1
Fetal Mineral Content Analysis
2
Tissue Iron Quantification Protocol
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Liver, kidney, spleen, and heart nonheme iron as well as serum iron were measured by using a colorimetric assay per the manufacturer’s protocol (Sekisui Diagnostics). Before sampling, tissues were pulverized in liquid nitrogen to reduce variation resulting from regional differences in tissue iron deposition. Complete blood counts were measured by using a HemaVet blood analyzer (Drew Scientific). The distribution of nonheme iron in deparaffinized formalin-fixed paraffin-embedded sections of the kidney, liver, and spleen was visualized by using Perls’ Prussian blue stain or diaminobenzidine-enhanced Perls’ stain. Slides were counterstained with Nuclear Fast Red solution (Vector) and visualized with a Nikon Eclipse E600 microscope and SPOT Imaging Software.
3
Iron Homeostasis Biomarkers in Lung Infections
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Full blood count and hemoglobin was assessed every 24 h through a hematocytometer (Siemens Advia 2021i, Erlangen, Germany). At the same time points, serum iron was measured using a colorimetric assay (Sekisui Diagnostics, Lexington, MA). Total iron-binding capacity (TIBC) was measured using a colorimetric assay (Pointe Scientific). Transferrin levels were calculated using the formula TIBC (μmol/L)/25.1. Transferrin saturation was calculated with the formula serum iron/(25.1 × total transferrin) × 100%. Ferritin (LsBio), hepcidin (LsBio), and interleukin 6 (IL-6) levels (Millipore Iberica, S.A., Madrid, Spain) were measured by enzyme-linked immunosorbent assay kits. Lung tissue was homogenized and cultured on agar plates at 37 °C with 5% CO2. The number of CFUs was counted the next day.
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