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Human hct116

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The Human HCT116 is a cell line derived from a human colorectal carcinoma. It is a commonly used model for the study of cancer biology and drug development.

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Lab products found in correlation

Sw480, by American Type Culture Collection (2 mentions) Ht29 crc, by American Type Culture Collection (1 mentions) Rpmi 1640 medium, by Thermo Fisher Scientific (1 mentions) Dld 1, by American Type Culture Collection (1 mentions) Bovine calf serum (bcs), by Greiner (1 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions) Rpmi 1640, by Lonza (1 mentions) Lim1215, by American Type Culture Collection (1 mentions) Mycoplasma detection kit, by Roche (1 mentions) Sw480, by Lonza (1 mentions) Celltiter 96 aqueous mts reagent, by Promega (1 mentions)

Close spelling variants of this product

HCT116 (3458 citations) HCT116 human colon cancer cells (12 citations) HCT116 human colorectal carcinoma cell line (7 citations) HCT116 human colorectal carcinoma cells (7 citations) HCT116 human colon carcinoma cells (6 citations) HCT116 human colorectal cancer cells (5 citations) HCT116 human colon cancer cell line (5 citations) Human HCT116 cells (3 citations) HCT116 human CRC cells (2 citations) HCT116 human colon adenocarcinoma cells (2 citations)

5 protocols using human hct116

1

Culturing Colorectal Cancer Cell Lines

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CT26 mouse CRC cell line, human HCT116, HCT116/RFP, and HT29 CRC cell lines were obtained from the American Type Culture Collection (ATCC) (Rockville, MD). Cells were cultured in RPMI and McCoy's 5a Medium and supplemented with 10% (v/v) fetal bovine serum (FBS), 100 U/mL penicillin, and 100 μg/mL streptomycin respectively (5% CO2, 37 °C).
Liu Z., Gray B.D., Barber C., Wan L., Furenlid L.R., Liang R., Li Z., Woolfenden J.M., Pak K.Y, & Martin D.R. (2021). PEGylated and non-PEGylated TCP-1 probes for imaging of colorectal cancer. Molecular imaging and biology, 25(1), 133-143.
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2

Murine and Human CRC Cell Lines

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Murine CT26 and human HCT116, DLD-1, HT29, and SW480 CRC cell lines were obtained from American Type Culture Collection (ATCC, Manassas, United States). All experiments were performed in RPMI 1640 medium (Thermo Fisher, Belgium) supplemented with 10% bovine calf serum (Greiner Bio-One, Belgium). Chemicals were obtained from Sigma-Aldrich (Antwerp, Belgium) unless otherwise stated.
de Mey S., Jiang H., Corbet C., Wang H., Dufait I., Law K., Bastien E., Verovski V., Gevaert T., Feron O, & De Ridder M. (2018). Antidiabetic Biguanides Radiosensitize Hypoxic Colorectal Cancer Cells Through a Decrease in Oxygen Consumption. Frontiers in Pharmacology, 9, 1073.
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3

Culturing and Characterizing CRC Cell Lines

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Human HCT116, LoVo, SW480, LIM1215, and SW48 CRC cancer cell lines were obtained from the American Type Culture Collection (ATCC) and authenticated by IRCCS “Azienda Ospedaliera Universitaria San Martino-IST Istituto Nazionale per la Ricerca sul Cancro, Genova,” (Italy). Human HCT116, LoVo, SW480, LIM1215, and SW48 CRC cancer cell lines were grown in RPMI- 1640 (Lonza), supplemented with 10% fetal bovine serum (gibco, purchased by thermofisher) and 1% penicillin/streptomycin. All cancer cells were grown in a humidified incubator with 5% of carbon dioxide and 95% air at 37 °C and were routinely screened for the presence of mycoplasma (Mycoplasma Detection Kit; Roche Diagnostics).
Ciardiello D., Blauensteiner B., Matrone N., Belli V., Mohr T., Vitiello P.P., Martini G., Poliero L., Cardone C., Napolitano S., De Falco V., Giunta E.F., Ciaramella V., Corte C.D., Barra G., Selvaggi F., Franco R., Marino F.Z., Cuomo A., Morgillo F., Troiani T., Sibilia M., Ciardiello F, & Martinelli E. (2021). Dual inhibition of TGFβ and AXL as a novel therapy for human colorectal adenocarcinoma with mesenchymal phenotype. Medical Oncology (Northwood, London, England), 38(3), 24.
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4

Colorectal Cancer Cell Line Treatment

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HCT116 human colorectal cancer cells were obtained from the American Type Culture Collection (CCL-247™, Rockville, MD, USA). HCT116 cells were incubated at 37℃ with 5% CO2 in RPMI 1640 supplemented with 10% fetal bovine serum, 2 mM L-glutamine, 2 U/mL penicillin G, and 2 mg/mL streptomycin. Cells were treated with PT and/or balsalazide in serum free medium, as indicated. In experiments involving caspase inhibition, cells were treated with a pan-caspase inhibitor (Z-VAD-FMK; 10 µM) for 1 hour prior to treatment with PT and balsalazide.
Kim H.Y., Kim S.L., Park Y.R., Liu Y.C., Seo S.Y., Kim S.H., Kim I.H., Lee S.O., Lee S.T, & Kim S.W. (2015). Balsalazide Potentiates Parthenolide-Mediated Inhibition of Nuclear Factor-κB Signaling in HCT116 Human Colorectal Cancer Cells. Intestinal Research, 13(3), 233-241.
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5

Colorectal Cancer Cell Proliferation Assay

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SW-480, HT-29, HCT-116 human colorectal cancer cells, and IEC-6 rat small intestine epithelial cells were purchased from American Type Culture Collection (ATCC, Manassas, VA) and grown in the L-15 or McCoy’s 5A medium supplemented with 10% FBS and 50 IU penicillin/streptomycin in a humidified atmosphere of 5% CO2 (100% air for SW-480 cells) at 37°C. For the proliferation assay, each type of cell was seeded in 96-well plates (5×103 cells/well) to adhere overnight. Various concentrations of PPD (5, 10, 20, 30, and 40 µM) then were administrated to the wells. Controls were treated with culture medium containing 0.1% DMSO. After 24, 48, and 72 hr, cell survival and growth were measured by the Cell Titer 96 Aqueous MTS Reagent (Promega, Madison, WI) according to the manufacturer’s protocol. All experiments were performed in triplicate and repeated three times, independently. The light absorbance was measured by using an automatic microplate reader (Epoch, Bio-Tek Instruments, Winooski, VT) at 490 nm (14 (link)). Data were expressed as a percentage versus control (vehicle set at 100%).
Zhang Z., Li Z., Wu X., Zhang C., Calway T., He T.C., Du W., Chen J., Wang C.Z, & Yuan C.S. (2014). TRAIL Pathway is Associated with Inhibition of Colon Cancer by Protopanaxadiol. Journal of pharmacological sciences, 127(1), 83-91.
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