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1 hydroxybenzotriazole hobt

Manufactured by Adamas
Sourced in China

1-hydroxybenzotriazole (HOBt) is a chemical compound that serves as a coupling agent in organic synthesis. It is used to facilitate the formation of amide bonds between carboxylic acids and amines. HOBt enhances the reactivity and increases the efficiency of the coupling reaction, making it a useful tool in the field of peptide synthesis and other organic chemistry applications.

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2 protocols using 1 hydroxybenzotriazole hobt

1

Polystyrene Resin Functionalization

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Chloromethylated polystyrene resin (PS-Cl) and D380 (PS-NH2) were purchased from Chemical Factory of Nankai University (Tianjin, China). Citric acid, trimethyl citrate, KI, NaH, tetrabutylammonium bromide (TBAB), 4-dimethylaminopyridine (DMAP), N,N-dicyclohexylcarbodiimide (DCC) and 1-hydroxybenzotriazole (HOBt) were purchased from Adamas Reagent Co., Ltd. The reagents and chemicals (analytic grade unless stated otherwise) were purchased from Sinopharm Chemical Reagent Co., Ltd and Tianjin Damao Chemical Reagent Co., Ltd (China).
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2

Theranostic Hg2+ Fluorescent Probe Synthesis

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The theranostic Hg2+ fluorescent probe, 3-formyl-7-diethylamino coumarin masked meso-dimercaptosuccinic acid (C-DMSA), was synthesized according to the literature procedures.11 (link) Chitosan (CS, Mw 50 kDa, 90.0% deacetylation degree) was purchased from JinQiao Biochemical Co. Ltd. (Shandong, China). Chitosan oligosaccharide (CSO, MW 5 kDa, 90.0% deacetylation degree) was obtained from JinQiao Biochemical Co. Ltd. (Shandong, People's
Republic of China). Vitamin E succinate (VES) was purchased from TCI Development Co. Ltd. (Shanghai, People's Republic of China). N-Hydroxysuccinimide (NHS) and 1-Hydroxybenzotriazole (HOBt) were purchased from Adamas Co. Ltd. Mercury perchlorate trihydrate was purchased from XianDing Biotechnology Co. Ltd. (Shanghai, People's Republic of China). All the other analytical chemicals and reagents were analytical grade. FBS, PBS, 0.25% (w/v) trypsin solution, penicillin-streptomycin and DMEM were purchased from Gibco BRL (Gaithersburg, MD, USA). IR-775 chloride, pentobarbital sodium and MTT were purchased from Sigma (St. Louis, USA). Hoechst 33258 was purchased from Beyotime Institute of Biotechnology (Shanghai, People's Republic of China).
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