Glyceryl monostearate purified powder (GMS) was purchased from Alfa Aesar (Ward Hill, MA). The surfactants; poloxamer 188 was obtained from Fisher Scientific (Hampton, NH), and polysorbate 80 (Tween® 80) from EMD Chemicals Inc., (Gibbstown, NJ). Gemcitabine was purchased from AK Scientific (Union City, CA). Pancreatic cancer MIA-PaCa-2 cells (K-Ras (G12C)) were bought from American Type Culture Collection (ATCC) (Manassas, VA). PPCL-46 46 is a patient-derived cell line (K-Ras (G12V)) obtained from Dr. Trevino’s lab with methods previously described (Pham et al., 2016 (link)). This cell line was derived from a 75-year-old, Caucasian female with a moderately differentiated, T3N1 pancreatic ductal adenocarcinoma who underwent pancreatoduodenectomy for her disease. All solvents used were of analytical grade.
Mia paca 2 cells k ras g12c
Manufactured by American Type Culture Collection
MIA-PaCa-2 cells (K-Ras (G12C)) are a cell line derived from a human pancreatic carcinoma. The cells harbor a mutation in the K-Ras gene, specifically the G12C substitution. This cell line is commonly used in research related to pancreatic cancer and the study of K-Ras-driven signaling pathways.
Automatically generated - may contain errors
2 protocols using mia paca 2 cells k ras g12c
1
Pancreatic Cancer Cell Line Generation
2
Investigating Pancreatic Cancer Cell Lines
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Gemcitabine Hydrochloride (GemHCl) was purchased from AK Scientific (Union City, CA). Pancreatic cancer MiaPaCa-2 cells (K-Ras (G12C)) were bought from American Type Culture Collection (ATCC) (Manassas, VA). PPCL-46 is a patient-derived cell line (K-Ras (G12V)) obtained from Dr. Trevino's laboratory with methods previously described (Pham et al., 2016 (link)). The PPCL-46 was derived from a 75-year-old, Caucasian female with a moderately differentiated, T3N1 pancreatic ductal adenocarcinoma who underwent pancreatoduodenectomy for her disease. All materials, both solvents and reagents of analytical grade were purchased from Sigma Aldrich (St. Louis, MO).
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