The RL95-2 (ATCC®CRL-1671™) and HEC-1-A (ATCC®HTB-112™) human endometrial carcinoma cell lines were purchased from the American Type Culture Collection (Manassas, VA, USA). RL95-2 cells were cultured in Dulbecco’s modified Eagle’s medium nutrient mixture F-12 (DMEM/F12) supplemented with 10% fetal bovine serum (FBS), 0.005 mg/mL insulin, and 1% penicillin–streptomycin (Thermo Fisher Scientific, Waltham, MA, USA). HEC-1-A cells were cultured in McCoy’s 5A medium supplemented with 10% FBS and 1% penicillin–streptomycin. Doxorubicin, cisplatin, sodium palmitate, 2′,7-dichlorofluorescein diacetate (DCFH-DA), propidium iodide (PI), and thiazolyl blue tetrazlium bromide (MTT) were obtained from Sigma Aldrich (St. Louis, MO, USA).
Rl95 2 atcc crl 1671
Manufactured by American Type Culture Collection
Sourced in United States
The RL95-2 (ATCC®CRL-1671™) is a cell line derived from a human endometrial carcinoma. It is cultivated using standard cell culture techniques.
Automatically generated - may contain errors
Lab products found in correlation
Penicillin streptomycin, by Thermo Fisher Scientific (4 mentions)
Doxorubicin, by Merck Group (2 mentions)
2 7 dichlorofluorescein diacetate dcfh da propidium iodide, by Merck Group (2 mentions)
Rl95 2, by Thermo Fisher Scientific (2 mentions)
Cisplatin, by Merck Group (2 mentions)
Dmem f12, by Thermo Fisher Scientific (2 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (2 mentions)
Thiazolyl blue tetrazlium bromide mtt, by Merck Group (1 mentions)
Sodium palmitate, by Merck Group (1 mentions)
Hec 1 a atcc htb 112, by American Type Culture Collection (1 mentions)
Tramadol, by Merck Group (1 mentions)
Dulbecco s modified eagle medium nutrient mixture f 12 dmem f 12, by Thermo Fisher Scientific (1 mentions)
Hec 1a, by Thermo Fisher Scientific (1 mentions)
Mccoy s 5a medium, by Thermo Fisher Scientific (1 mentions)
Mitosox red, by Thermo Fisher Scientific (1 mentions)
Thiazolyl blue tetrazolium bromide mtt, by Merck Group (1 mentions)
2 protocols using rl95 2 atcc crl 1671
1
Endometrial Carcinoma Cell Lines for Cytotoxicity Assays
2
Endometrial Cancer Cell Lines Characterization
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Human endometrial adenocarcinoma cell lines RL95-2 (ATCC®CRL-1671™) and HEC-1-A cells (ATCC®HTB-112™) were obtained from the American Type Culture Collection (ATCC; Manassas, VA, USA). For the cell culture experiments, we cultured RL95-2 and HEC-1-A cells in Dulbecco’s Modified Eagle Medium Nutrient Mixture F-12 (DMEM/F12) and McCoy’s 5A medium supplemented with 10% fetal bovine serum (FBS) and 1% penicillin–streptomycin (Thermo Fisher Scientific, Waltham, MA, USA), respectively. Tramadol, doxorubicin, cisplatin, 2′,7-dichlorofluorescein diacetate (DCFH-DA), propidium iodide (PI), and thiazolyl blue tetrazolium bromide (MTT) were obtained from Sigma Aldrich (Sigma Aldrich; St. Louis, MO, USA). MitoSOX Red was obtained from Invitrogen (Invitrogen; Waltham, MA, USA).
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