Peroxidase conjugated anti rabbit igg antibody

Manufactured by Jackson ImmunoResearch

Sourced in Panama

Peroxidase-conjugated anti-rabbit IgG antibody is a laboratory reagent used to detect and quantify the presence of rabbit immunoglobulin G (IgG) in various assays and experimental procedures. The antibody is conjugated with the enzyme peroxidase, which catalyzes a colorimetric reaction upon the addition of a suitable substrate, enabling the visualization and quantification of the target rabbit IgG.

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Rabbit anti-IgG (2 citations) Rabbit IgGs (2 citations)

4 protocols using peroxidase conjugated anti rabbit igg antibody

Western Blotting of Cerebral Cortex Proteins

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Western blotting of brain tissues were performed as previously described [18 (link)]. The cerebral cortexes of mice were homogenized in lysis buffer (50 mM HEPES, pH7.2, 150 mM NaCl, 5 mM MgCl₂) containing cOmplete™ EDTA-free protease inhibitor (Roche Diagnostics, Basel, Switzerland)) and centrifuged at 1,000 g for 5 min. The tissue lysates (20 μg of protein) were separated on SDS-PAGE (10% acrylamide gel). The separated proteins were transferred to a polyvinylidene difluoride membrane (Pall Life Sciences, Port Washington, NY). The membrane was blocked with 5% skim milk and incubated with an anti-ERK1/2 antibody (1:1000 dilution, BRID: AB_330744, Cell Signaling Technology, Danvers, MA), an anti-phospho-ERK1/2 antibody (1:1000 dilution, BRID: AB_331646, Cell Signaling Technology) or an anti-β-actin polyclonal antibody (1:1000 dilution, BRID: AB_476693, Sigma-Aldrich, St Louis, MO) overnight at 4 °C. The immunoreactive bands were visualized using a peroxidase-conjugated anti-rabbit IgG antibody (1:10000 dilution, BRID: AB_2337943, Jackson ImmunoResearch Laboratories, West Grove, PA) and the ECL Western Blotting Detection System (GE Healthcare Bio-Sciences, Piscataway, NJ).

Komenoi S., Suzuki Y., Asami M., Murakami C., Hoshino F., Chiba S., Takahashi D., Kado S, & Sakane F. (2019). Microarray analysis of gene expression in the diacylglycerol kinase η knockout mouse brain. Biochemistry and Biophysics Reports, 19, 100660.

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Investigating EGFR Signaling Pathways

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CTF was purchased from American Diagnostica, Inc. [Greenwich, CT]. EGF was purchased from Sigma Chemical Co. [St. Louis, MO]. AG1478 and CRM197 were purchased from Calbiochem [La Jolla, CA]. GM6001 was purchased from Chemicon International, Inc. [Temecula, CA]. CRM197, TAPI-0 and TAPI-1 were purchased from Biomol [Hamburg, Germany]. The anti–HB-EGF antibody was purchased from R&D Systems, Inc. [Minneapolis, MN]. The anti-EGFR antibody [151-IgG] developed by Dr. Ann Hubbard was obtained from the Developmental Studies Hybridoma Bank, developed under the auspices of the National Institute of Child Health and Human Development and maintained by The University of Iowa [Department of Biological Sciences, Iowa City, IA]. Pertussis toxin, GP2A, AG1478, PP3, PP2, H7, GF109203X, BAPTA-AM, NAC, apocynin and DPI were purchased from Calbiochem [La Jolla, CA]. Peroxidase-conjugated anti-rabbit IgG antibody [raised in goat] was purchased from Jackson ImmunoResearch Laboratories, Inc. [West Grove, PA]. Peroxidase-conjugated anti-mouse IgG antibody [raised in goat] was purchased from Biorad Laboratories [Hercules, CA]. Phospho- EGFR [Y1068], Total EGFR antibodies were obtained from Cell Signaling Technology, Inc. [Beverley, MA]. Dulbecco’s modified Eagle minimal essential medium [DMEM] and Dulbecco’s phosphate-buffered saline were purchased from Mediatech [Herndon, VA].

Duru E.A., Fu Y, & Davies M.G. (2014). PROTEASE MEDIATED HUMAN SMOOTH MUSCLE CELL PROLIFERATION BY UROKINASE REQUIRES EPIDERMAL GROWTH FACTOR RECEPTOR TRANSACTIVATION BY TRIPLE MEMBRANE SIGNALING. The Journal of surgical research, 192(2), 254-262.

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Diacylglycerol Kinase Protein Expression

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The testis, ovary and uterus from 10- to 12-week-old male and female mice were homogenized in lysis buffer (50 mM HEPES, pH7.2, 150 mM NaCl, and 5 mM MgCl₂) containing complete EDTA-free protease inhibitor cocktail (Roche Diagnostics) and centrifuged at 1,000 × g for 5 min. The protein concentration in the supernatants was determined using a bicinchoninic acid protein assay kit (Thermo Scientific, Hudson, NH, USA). The tissue lysates (30 μg of protein) were separated on SDS-PAGE, and the separated proteins were transferred to a polyvinylidene difluoride membrane (Pall Life Sciences, Port Washington, NY, USA). The membrane was blocked with 5% skim milk and incubated with an anti-DGKδ polyclonal antibody [15 (link)] and an anti-DGKη polyclonal antibody (ProteinTech Group, Chicago, IL, USA) overnight at 4°C. The immunoreactive bands were visualized using a peroxidase-conjugated anti-rabbit IgG antibody (Jackson ImmunoResearch Laboratories, West Grove, PA, USA) and the ECL Western Blotting Detection System (GE Healthcare Bio-Sciences, Piscataway, NJ, USA).

Shionoya T., Usuki T., Komenoi S., Isozaki T., Sakai H, & Sakane F. (2015). Distinct expression and localization of the type II diacylglycerol kinase isozymes δ, η and κ in the mouse reproductive organs. BMC Developmental Biology, 15, 6.

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Inhibition of MAPK Signaling Pathways

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suPAR and ATF were purchased from American Diagnostica, Inc. (Greenwich, CT). fMLP, Pertussis toxin (Gαi inhibitor, 100ng/ml), Phosphatidylinositol-specific-phospholipase C (100µg/mL), PD98059 (ERK inhibitor, 25µM) SB203580 (p38^MAPK inhibitor, 10µM), SP600125 (JNK inhibitor, 1µM), EGFR inhibitor ( AG1478, 10µM), ε-aminocaproic acid (EACA; plasmin inhibitor, 100µM), aprotinin (plasmin inhibitor, 100 units/ml) and GM6001 (MMP inhibitor, 10nM) were purchased from Sigma Chemical Co. (St. Louis, MO). Peroxidase-conjugated anti-rabbit IgG antibody (raised in goat) and peroxidase-conjugated anti-mouse IgG antibody (raised in goat) were purchased from Jackson ImmunoResearch Laboratories, Inc. (West Grove, PA). Phospho-ERK1/2 antibody was purchased from Promega, Inc. (Madison, WI). Total ERK 1/2 antibody was purchased from BD Transduction Laboratories (Lexington, KY). Phospho-p38^MAPK and phospho-JNK antibodies were purchased from Biosource (Camarillo, CA). Total p38^MAPK and JNK antibodies were purchased from Cell Signaling (Beverley, MA). Dulbecco’s minimal essential medium (DMEM) and Dulbecco’s phosphate buffered saline (dPBS) were purchased from Corning Cellgro (Corning, NY).

Duru E.A., Fu Y, & Davies M.G. (2015). ROLE OF FORMIC RECEPTORS IN SOLUBLE UROKINASE RECEPTOR INDUCED HUMAN VASCULAR SMOOTH MUSCLE MIGRATION. The Journal of surgical research, 195(2), 396-405.

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