Mc4l2 cell line

CNTs with <99% purity (SWCNT: 1–2 nm length, MWCNT: 20–30 nm length) were purchased from Notrino Co (St. Sadeghiye, Tehran, Iran). The MC4L2 cell line was purchased from Iranian Biological Resource Center (Tehran, Iran). Dulbecco’s Modified Eagle Medium (DMEM) cell culture and FBS were purchased from Scotland (Gibco, Scotland). MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) powder and Doxorubicin were purchased from Sigma Co. Annexin kit was purchased from Iq products, Poland. Ketamine and Xylazine were purchased from (Sigma Aldrich Co, USA). The BALB/C mouse was purchased from Iran Pasteur Institute (Tehran, Iran). TRIzol solution and 2× Real Rime SYBER GREEN Master Mix were purchased from Invitrogen, USA. The first standard cDNA synthesis kit and nuclease-free water were purchased from Representing of Fementas Co. (USA).

MC4L2 cell line (mice estrogen receptor-positive breast carcinoma cells, obtained from Iranian Biological Resource Center, Tehran, Iran) was cultured in DMEM medium, supplemented with FBS (10%), penicillin (100 units/mL) and streptomycin (100 μg/mL). The cells were grown in a CO₂ incubator (Memmert, Germany) at 37 °C.