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Rabbit anti gs

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Rabbit anti-GS is a primary antibody that binds to the glutamine synthetase (GS) protein. It is commonly used in various research and diagnostic applications that involve the detection and analysis of the GS protein.

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Lab products found in correlation

Lsm 710, by Zeiss (2 mentions) Rabbit anti synaptophysin, by Abcam (2 mentions) Goat anti lyve 1, by R&D Systems (1 mentions) Rabbit anti ck19, by Proteintech (1 mentions) Sma cy3 clone 1a4, by Merck Group (1 mentions)

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Rabbit anti- (2 citations)

3 protocols using rabbit anti gs

1

Immunohistochemical Analysis of Retinal Protein

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Retinas were freshly dissected and immediately placed in 10% formalin overnight. Relief cuts were made and the retinas were embedded in 5% agarose. Using a vibratome, 150 μm transverse sections were cut and the sections were floated in PBS. After blocking in 1% bovine serum albumin, 0.5% Triton X-100, and 2% normal donkey serum for 2–3 hours, sections were incubated in primary antibody overnight at 4° C. After washing in PBS, secondary antibodies were applied at room temperature for 1 hour. Sections were again washed and then mounted for confocal microscopy (LSM710, Carl Zeiss). Antibodies were as follows: 3R10 mouse anti-RS17 (link) (gift of Professor Robert Molday, 1:5); rabbit anti-GS (Sigma, 1:1000); rabbit anti-synaptophysin (abcam, 1:1000).
Byrne L.C., Öztürk B.E., Lee T., Fortuny C., Visel M., Dalkara D., Schaffer D.V, & Flannery J.G. (2014). Retinoschisin gene therapy in photoreceptors, Müller glia, or all retinal cells in the Rs1h−/− mouse. Gene therapy, 21(6), 585-592.
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2

Immunofluorescence Staining of Lymphatic Markers

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The following antibodies were used: goat anti-LYVE-1 (polyclonal, R&D, AF2089), rat anti-PECAM-1 (clone 5D2.6 and IG5.1, provided by D. Vestweber, Max Planck Institute for Molecular Biomedicine Munster), rabbit anti-LYVE-1 (polyclonal, Reliatech), goat anti-LDLR (polyclonal, R&D, AF2255), rabbit anti-GS (polyclonal, Sigma-Aldrich, G2781), rabbit anti-CK19 (polyclonal, proteintech, 14965-1-AP), mouse anti-smooth muscle actin (SMA)-Cy3 (clone 1A4, Sigma), rat anti-Lyve-AlexaFluor594 (clone 223322, R&D), secondary antibodies coupled to AlexaFluor488, 568 and 647 (Invitrogen).
Bobe S., Beckmann D., Klump D.M., Dierkes C., Kirschnick N., Redder E., Bauer N., Schäfers M., Erapaneedi R., Risse B., van de Pavert S.A, & Kiefer F. (2022). Volumetric imaging reveals VEGF-C-dependent formation of hepatic lymph vessels in mice. Frontiers in Cell and Developmental Biology, 10, 949896.
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3

Immunohistochemical Analysis of Retinal Protein

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Retinas were freshly dissected and immediately placed in 10% formalin overnight. Relief cuts were made and the retinas were embedded in 5% agarose. Using a vibratome, 150 μm transverse sections were cut and the sections were floated in PBS. After blocking in 1% bovine serum albumin, 0.5% Triton X-100, and 2% normal donkey serum for 2–3 hours, sections were incubated in primary antibody overnight at 4° C. After washing in PBS, secondary antibodies were applied at room temperature for 1 hour. Sections were again washed and then mounted for confocal microscopy (LSM710, Carl Zeiss). Antibodies were as follows: 3R10 mouse anti-RS17 (link) (gift of Professor Robert Molday, 1:5); rabbit anti-GS (Sigma, 1:1000); rabbit anti-synaptophysin (abcam, 1:1000).
Byrne L.C., Öztürk B.E., Lee T., Fortuny C., Visel M., Dalkara D., Schaffer D.V, & Flannery J.G. (2014). Retinoschisin gene therapy in photoreceptors, Müller glia, or all retinal cells in the Rs1h−/− mouse. Gene therapy, 21(6), 585-592.
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