Vero tmprss2 cells

Manufactured by Japanese Collection of Research Bioresources Cell Bank

Vero/TMPRSS2 cells are a cell line derived from the kidney of an African green monkey. They express the TMPRSS2 protein, which is a host cell serine protease that is involved in the priming of the SARS-CoV-2 spike protein, enabling viral entry into host cells. These cells can be used for research purposes in the study of SARS-CoV-2 and other viral infections.

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Lab products found in correlation

Vero e6, by American Type Culture Collection (4 mentions) Hek293t, by American Type Culture Collection (4 mentions) A549 ace2 tmprss2 cells, by InvivoGen (3 mentions) A549 hace2tpsa, by InvivoGen (2 mentions) Hyclone fetalclone 2, by Cytiva (2 mentions) Geneticin, by Thermo Fisher Scientific (2 mentions) Hepes, by Thermo Fisher Scientific (2 mentions) Hepes, by Corning (2 mentions) Thp 1 cells, by Japanese Collection of Research Bioresources Cell Bank (1 mentions) Thp 1 dual reporter cells, by InvivoGen (1 mentions) Hct 8 cells, by American Type Culture Collection (1 mentions) Calu 3 cells, by American Type Culture Collection (1 mentions) Mrc 5 cells, by American Type Culture Collection (1 mentions) 293t cells, by RIKEN BioResource Center (1 mentions) Vero cells, by RIKEN BioResource Center (1 mentions) Hyclone, by Thermo Fisher Scientific (1 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions) Calu 3, by American Type Culture Collection (1 mentions) Hygromycin gold, by InvivoGen (1 mentions) Puromycin, by InvivoGen (1 mentions)

Close spelling variants of this product

Vero cells

6 protocols using vero tmprss2 cells

Cell Line Maintenance for Virus Research

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293T cells (human embryonic kidney cell line) and Vero cells (African green monkey kidney cell line) were obtained from the RIKEN BioResource Center Cell Bank. Calu-3 cells (human bronchial epithelial cell line), MRC5 cells (human fetal lung fibroblast cell line) and HCT8 cells (human rectal adenocarcinoma cell line) were obtained from the American Type Culture Collection (ATCC). THP-1 cells and Vero/TMPRSS2 cells⁵³ (link) were obtained from the Japanese Collection of Research Bioresources Cell Bank. 293T, Calu-3 and MRC5 cells were maintained in Dulbecco’s modified Eagle’s Medium (DMEM) containing 10% fetal calf serum (FCS). HCT8 and THP-1 cells were maintained in RPMI-1640 medium with 10% FCS. Vero cells and Vero/TMPRSS2 cells were maintained in DMEM containing 10% FCS. 1 mg/mL G418 (Invivogen) was added to the growth medium for Vero/TMPRSS2 cells. THP-1-dual reporter cells, THP-1-dual KO-RIG-I cells and THP-1-dual KO-MDA5 cells were purchased from InvivoGen and maintained according to the manufacturer’s instructions.

Arai Y., Yamanaka I., Okamoto T., Isobe A., Nakai N., Kamimura N., Suzuki T., Daidoji T., Ono T., Nakaya T., Matsumoto K., Okuzaki D, & Watanabe Y. (2022). Stimulation of interferon-β responses by aberrant SARS-CoV-2 small viral RNAs acting as retinoic acid-inducible gene-I agonists. iScience, 26(1), 105742.

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Cell Line Cultivation Protocol

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All cell lines: Vero E6 (ATCC CRL-1586), HEK-293T (ATCC CRL-3216), Vero-TMPRSS2 cells (JCRB Cell Bank JCRB1819), and A549-ACE2-TMPRSS2 cells (Invivogen a549-hace2tpsa) were grown according to the manufacturer’s instructions.

Lubinski B., Frazier L.E., Phan M.V., Bugembe D.L., Cunningham J.L., Tang T., Daniel S., Cotten M., Jaimes J.A, & Whittaker G.R. (2022). Spike Protein Cleavage-Activation in the Context of the SARS-CoV-2 P681R Mutation: an Analysis from Its First Appearance in Lineage A.23.1 Identified in Uganda. Microbiology Spectrum, 10(4), e01514-22.

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Cell Line Cultivation Protocol

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Cell Culture Conditions for SARS-CoV-2 Research

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All cell lines were grown at 37°C with 5% CO₂. Vero E6 (ATCC CRL-1586) and Hek293T (ATCC CRL-3216) cells were grown in Dulbeco’s Modified Eagle’s Medium (DMEM)(Corning) with 10% Hyclone™ FetalClone® II (Cytiva), and 1% HEPES (Corning). Vero TMPRSS2 cells (JCRB Cell Bank JCRB1819) were grown in DMEM, 10% Hyclone, 1% HEPES, and 1% Geneticin (Gibco). Calu3 (ATCC HTB-55) cells were grown in Eagle’s Minimum Essential Medium (EMEM) (Corning) with 10% Fetal Bovine Serum (FBS)(Gibco).

Lubinski B., Fernandes M.H., Frazier L., Tang T., Daniel S., Diel D.G., Jaimes J.A, & Whittaker G.R. (2021). Functional evaluation of the P681H mutation on the proteolytic activation of the SARS-CoV-2 variant B.1.1.7 (Alpha) spike. iScience, 25(1), 103589.

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Cell Culture Conditions for SARS-CoV-2 Research

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All cell lines were grown at 37°C with 5% CO₂. Vero E6 (ATCC CRL-1586) and Hek293T (ATCC CRL-3216) cells were grown in Dulbeco’s Modified Eagle’s Medium (DMEM)(Corning) with 10% HyClone^™ FetalClone® II (Cytiva), and 1% HEPES (Corning). Vero TMPRSS2 cells (JCRB Cell Bank JCRB1819) were grown in DMEM, 10% Hyclone, 1% HEPES, and 1% Geneticin (Gibco). A549 ACE2 TMPRSS2 cells (Invivogen a549-hace2tpsa) were maintained in DMEM with 10% FBS, 0.5 μg/ml of Puromycin (Invivogen ant-pr-1) and 300 μg/ml of Hygromycin Gold (Invivogen ant-hg-1).

Lubinski B., Frazier L.E., Phan M.V., Bugembe D.L., Cunningham J.L., Tang T., Daniel S., Cotten M., Jaimes J.A, & Whittaker G.R. (2022). Spike protein cleavage-activation in the context of the SARS-CoV-2 P681R mutation: an analysis from its first appearance in lineage A.23.1 identified in Uganda. bioRxiv.

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SARS-CoV-2 Virus Propagation and Titration

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SARS-CoV-2 was isolated and propagated as previously described and used for the infection experiment (50 (link)). The virus used for rechallenge was original virus stock propagated in Vero/TMPRSS2 cells cultured with 2% fetal bovine serum (FBS) in Dulbecco’s modified Eagle’s medium (DMEM) at 37 °C for 3 d. Vero/TMPRSS2 cells were obtained from JCRB Cell Bank and were maintained in 5% FBS DMEM supplemented with 1 mg/mL G418 (Roche). VeroE6/TMPRSS2 (P13) cells were obtained from JCRB Cell Bank and were maintained in 7% FBS DMEM supplemented with 1 mg/mL G418. All cells were cultured at 37 °C in a humidified 5% CO₂ atmosphere. The TCID₅₀ of the virus was measured using VeroE6/TMPRSS2 cells.

Urano E., Okamura T., Ono C., Ueno S., Nagata S., Kamada H., Higuchi M., Furukawa M., Kamitani W., Matsuura Y., Kawaoka Y, & Yasutomi Y. (2021). COVID-19 cynomolgus macaque model reflecting human COVID-19 pathological conditions. Proceedings of the National Academy of Sciences of the United States of America, 118(43), e2104847118.

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