Live red blood cell confocal imaging, before and after freezing with cryoprotectants, was undertaken using a Zeiss LSM 710 inverted microscope, equipped with three photo-multiplier detectors (GaAsP, multialkali, and BiG.2) and a multi-channel spectral imaging detector. Red blood cells (diluted 500-fold) were placed on MatTek glass bottom dishes (no. 1.5, 35 mm) and brightfield images were taken using a C-Apochromat 63x/1.20 W Korr M27 objective lens and ×3.0 zoom. Z-stacks were taken every z = 0.3 μm until the whole cell was imaged. Zeiss ZEN (black edition) 2.3 lite was utilized for image collection and maximum intensity projection processing.
Zen black edition 2.3 lite
Manufactured by Zeiss
ZEN (black edition) 2.3 lite is a software package developed by Zeiss for microscopy applications. It provides a user interface and tools for image acquisition, processing, and analysis. The software is designed to work with Zeiss microscope hardware, but can also be used with other imaging systems.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using zen black edition 2.3 lite
1
Cryoprotectant Effects on Red Blood Cells
2
Live Red Blood Cell Imaging
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Live red blood cell confocal imaging,
before and after freezing with cryoprotectants, was undertaken using
a Zeiss LSM 710 inverted microscope, equipped with three photomultiplier
detectors (GaAsP, multialkali, and BiG.2) and a multichannel spectral
imaging detector. Red blood cells (diluted 500-fold) were placed on
MatTek glass bottom dishes (no. 1.5, 35 mm) and brightfield images
were taken using a C-Apochromat 63x/1.20 W Korr M27 objective lens
and ×3.0 zoom. Z-stacks were taken every z =
0.3 μm until the whole cell was imaged. Zeiss ZEN (black edition)
2.3 lite was utilized for image collection and maximum intensity projection
processing.
before and after freezing with cryoprotectants, was undertaken using
a Zeiss LSM 710 inverted microscope, equipped with three photomultiplier
detectors (GaAsP, multialkali, and BiG.2) and a multichannel spectral
imaging detector. Red blood cells (diluted 500-fold) were placed on
MatTek glass bottom dishes (no. 1.5, 35 mm) and brightfield images
were taken using a C-Apochromat 63x/1.20 W Korr M27 objective lens
and ×3.0 zoom. Z-stacks were taken every z =
0.3 μm until the whole cell was imaged. Zeiss ZEN (black edition)
2.3 lite was utilized for image collection and maximum intensity projection
processing.
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