Gas chromatography–mass spectrometry analysis was performed as previously described (Ser et al., 2015b (link)). The equipment used was Agilent Technologies 6980N (GC) equipped with 5979 Mass Selective Detector (MS), HP-5MS (5% phenyl methyl siloxane) capillary column of dimensions 30.0 m × 250 μm × 0.25 μm and used helium as carrier gas at 1 mL/min. The column temperature was programmed initially at 40°C for 10 min, followed by an increase of 3°C/min to 250°C and was kept isothermally for 5 min. The MS was operating at 70 eV. The constituents were identified by comparison of their mass spectral data with those from NIST 05 Spectral Library.
5979 mass selective detector ms
Manufactured by Agilent Technologies
The 5979 Mass Selective Detector (MS) is a lab equipment product from Agilent Technologies. It is a sensitive and selective detector for gas chromatography (GC) systems, designed to identify and quantify chemical compounds in complex samples.
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3 protocols using 5979 mass selective detector ms
1
GC-MS Analysis of Microbial Metabolites
2
GC-MS Analysis of Compounds
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Gas chromatography-mass spectrometry (GC–MS) analysis was conducted based on previously developed protocol by Panigrahi et al. [27 (link)] with slight modifications. The Agilent Technologies 6980N (GC) equipped with 5979 Mass Selective Detector (MS), HP-5MS (5% phenyl methyl siloxane) capillary column of dimensions 60.0 m × 250 μm × 0.25 μm and helium as carrier gas at 1 mL/min were used for the analysis. The column temperature was maintained initially at 50 °C for 5 min, followed by an increase of 10 °C/min to 250 °C and was kept isothermal for 5 min. The MS was operating at 70 eV. The constituents were identified by comparison of their mass spectral data with those from W9N11 Spectral Library.
3
GC-MS Analysis of Metabolites
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Gas chromatography-mass spectrometry (GC–MS) analysis was conducted based on our previously developed protocol with slight modifications (Supriady et al., 2015 (link)). The Agilent Technologies 6980N (GC) equipped with 5979 Mass Selective Detector (MS), HP-5MS (5% phenyl methyl siloxane) capillary column of dimensions 30.0 m × 250 μm × 0.25 μm and helium as carrier gas at 1 mL/min were used for the analysis. The column temperature was maintained initially at 40°C for 10 min, followed by an increase of 3°C/min to 250°C and was kept isothermal for 5 min. The MS was operating at 70 eV. The constituents were identified by comparison of their mass spectral data with those available from W9N11 MS library.
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