Smaller regions of eyecups processed as described above, which included the retina, were emersion fixed in Karnovsky's fixative (2.5% glutaraldehyde and 2% paraformaldehyde in 200 mM Cacodylate buffer, pH 7.4) overnight at 4°C. The tissue was then post-fixed in 1% osmium tetroxide in phosphate buffer, dehydrated, and embedded in Epon epoxy. Sections (60–90 nm) were cut and stained with 50% ethanoic uranyl acetate and Reynold's lead citrate and viewed using a Phillips CM120 transmission electron microscope (FEI Company, Hillsboro, OR, USA).
Phillips cm120 transmission
Manufactured by Thermo Fisher Scientific
Sourced in United States
The Phillips CM120 transmission is a core laboratory instrument used for high-resolution transmission electron microscopy (TEM) analysis. It is designed to provide researchers with detailed structural and compositional information about a wide range of samples at the nanoscale level.
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Lab products found in correlation
2 protocols using phillips cm120 transmission
1
Ultrastructural Analysis of Retinal Tissue
2
Ultrastructural Analysis of Mitochondria-ER Interface
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Enucleated eyes were fixed in 4% paraformaldehyde in 0.1M phosphate buffer (PB) for 5 min, after which the anterior chambers and lenses were dissected away from each eyecup. A small region of the eyecup was then removed and placed in 2.5% glutaraldehyde +2% paraformaldehyde in 0.1M PB (pH 7.4) overnight at 4°C. Tissues were postfixed in 1% osmium tetroxide in PB, dehydrated in ethanol, and embedded in Epon epoxy. Sections (60–90 nm) were cut, stained with 50% ethanoic uranyl acetate and Reynold’s lead citrate, and viewed using a Phillips CM120 transmission electron microscope (FEI Company, Hillsborn, OR). The interface length (threshold distance between 20 and 100 nm) (Csordas et al., 2018 (link)) and the distance between the mitochondria and the ER were measured using ImageJ software.
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