Advance 2 600 mhz spectrometer

Manufactured by Bruker

Sourced in United States, Germany

The Bruker Advance II 600-MHz spectrometer is a high-performance nuclear magnetic resonance (NMR) instrument. It is designed to analyze the chemical structure and properties of various materials through the application of strong magnetic fields and radio frequency pulses. The core function of this spectrometer is to provide accurate and reliable data for researchers and scientists working in fields such as chemistry, biology, and materials science.

Automatically generated - may contain errors

Lab products found in correlation

Topspin 3, by Bruker (1 mentions) Topspin 2, by Bruker (1 mentions) Ft ir 4100 spectrophotometer, by Jasco (1 mentions) 60 f254 plate, by Merck Group (1 mentions) F 7000 spectrofluorimeter, by Hitachi (1 mentions) Cary 50 uv vis spectrometer, by Agilent Technologies (1 mentions)

Close spelling variants of this product

Advance 600 MHz spectrometer Advance 600 MHz Advance 600 spectrometer 600 MHz spectrometer Advance II spectrometer Advance 600 Advance spectrometer Advance II Bruker spectrometers

6 protocols using advance 2 600 mhz spectrometer

Structural Analysis of Heparin by NMR

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

One-dimensional (1D) ¹H and ¹³C NMR spectra were obtained at 600
MHz on ²H₂O exchanged heparin (20 mg/mL in
²H₂O) using a Bruker Advance II 600 MHz spectrometer (Bruker
BioSpin, Billerica, Massachusetts) with Topspin 3.2 software (Bruker BioSpin) for signal
integration. Two-dimensional HSQC-NMR spectra were obtained at 800 MHz on
²H₂O exchanged heparin (20 mg/mL in ²H₂O)
using a Bruker Advance II 800 MHz spectrometer.^{10 (link)}

Chen J., Yu Y., Fareed J., Hoppensteadt D., Jeske W., Kouta A., Jin C., Jin Y., Yao Y., Xia K., Zhang F., Chen S., Ye X, & Linhardt R.J. (2019). Comparison of Low-Molecular-Weight Heparins Prepared From Ovine Heparins With Enoxaparin. Clinical and Applied Thrombosis/Hemostasis, 25, 1076029619840701.

+ Open protocol

+ Expand

Heparin NMR Characterization Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols

One-dimensional (1D) proton and carbon NMR spectra were obtained at 600 MHz n D 2 O exchanged heparin (20 mg/mL in D 2 O) using a Bruker Advance II 600 MHz spectrometer (Bruker Bio-Spin, Billerica, Massachusetts) with Topspin 2.1.6 software (Bruker Bio-Spin) for signal integration. Twodimensional HSQC-NMR spectra were obtained at 800 MHz on D 2 O exchanged heparin (20 mg/mL in D 2 O) using a Bruker Advance II 600 MHz spectrometer. 12 Detailed experimental parameters are presented in the supporting information.

Liu X., St Ange K., Fareed J., Hoppensteadt D., Jeske W., Kouta A., Chi L., Jin C., Jin Y., Yao Y, & Linhardt R.J. (2017). Comparison of Low-Molecular-Weight Heparins Prepared From Bovine Heparins With Enoxaparin. Clinical and applied thrombosis/hemostasis : official journal of the International Academy of Clinical and Applied Thrombosis/Hemostasis, 23(6).

+ Open protocol

+ Expand

Silica-based Network Characterization

Check if the same lab product or an alternative is used in the 5 most similar protocols

Fourier Transform Infrared Spectroscopy (FTIR) was performed after complete hydrolysis signifying the existence of ethanol and silanol groups. The method signifies that the OH as well as the O–Si units were connected to a specific silicon atom, enhancing the silanol groups of Si–O–Si bonds permitting production of a 3D network.
The experimental specimens were also evaluated using micro-Raman spectroscopy using a beam intensity of 10 mW and an excitation wavelength of 633 nm. A randomly selected site was evaluated with a 10-acquisition time within the region of 100–3200 cm⁻¹. NMR (29Si CP-MAS solid-state NMR spectrum) was collected using Bruker Advance II 600 MHz spectrometer (Billerica, MA, USA).

Bapat R.A., Libat R., Yuin O.S., Parolia A., Ilyas M.S., Khan A.S., Kay M.K., Pichika M.R., Saxena K., Seow L.L., Sidhu P, & Daood U. (2023). Antimicrobial FiteBac® K21 promotes antimicrobial Potency and wound healing. Heliyon, 9(8), e19282.

+ Open protocol

+ Expand

Spectroscopic Characterization of Novel Compounds

Check if the same lab product or an alternative is used in the 5 most similar protocols

Melting points were determined in open capillary tubes using a Mettler 9100 electrothermal melting point apparatus and were uncorrected. IR spectra were recorded using a JASCO FTIR-4100 spectrophotometer. UV spectra were measured in MeOH using a UV-160 IPC UV-visible dual-beam spectrophotometer. The ¹H and ¹³C NMR spectra were obtained on a Bruker Advance II 600-MHz spectrometer operating at 600 and 150 MHz, respectively. Both ¹H and ¹³C NMR spectra were recorded in methanol-d₄, and the chemical shift values were expressed in δ (ppm) relative to the internal standard TMS. For the ¹³C NMR spectra, spectral editing was determined by DEPT. 2D NMR data were obtained using the standard pulse sequence of the Bruker 600 for COSY, HSQC and HMBC. Low resolution EIMS were obtained using a double-focusing magnetic sector mass spectrometer (GS-MS DFS/Thermo).

Abaza M.S., Orabi K.Y., Al-Quattan E, & Al-Attiyah R.J. (2015). Growth inhibitory and chemo-sensitization effects of naringenin, a natural flavanone purified from Thymus vulgaris, on human breast and colorectal cancer. Cancer Cell International, 15, 46.

+ Open protocol

+ Expand

Thin Layer Chromatography and Purification

Check if the same lab product or an alternative is used in the 5 most similar protocols

Thin layer chromatography (TLC) analysis was carried out on a 0.2 mm pre-coated aluminum sheet 60 F₂₅₄ plate (Merck Ltd., Darmstadt, Germany) with the mixture of hexane/ethyl acetate/formic acid (80:20:2, v/v/v). The compounds were detected using a solution of 10 g of Ce(SO₄)₂ and 20 g of phosphomolibdenic acid in 1 L of 10% H₂SO₄ followed by heating. The products were purified using column chromatography (CC) on silica gel (230–400 mesh). The solvent ratio was gradients of hexane/ether (2:1 next 9:1), hexane/ethyl acetate/formic acid (30:1:1.2 then 30:20:1). Each fraction was collected and monitored by TLC. Each pure product was procured following solvent removal. Spectroscopic analysis in the range of Nuclear Magnetic Resonance (¹H NMR, ¹³C NMR, COSY, HSQC) was recorded on a Bruker Advance II 600 MHz spectrometer (Bruker, Rheinstetten, Germany). Samples were dissolved in CDCl₃ and the chemical shifts of detected signals were referenced to the signals of residual solvent (δH = 7.26, δC = 77.00).

Szczepańska P., Rychlicka M., Groborz S., Kruszyńska A., Ledesma-Amaro R., Rapak A., Gliszczyńska A, & Lazar Z. (2023). Studies on the Anticancer and Antioxidant Activities of Resveratrol and Long-Chain Fatty Acid Esters. International Journal of Molecular Sciences, 24(8), 7167.

+ Open protocol

+ Expand

Spectroscopic Analysis of HTCC-FITC Interaction with SARS-CoV-2 and MERS-CoV Spike Proteins

Check if the same lab product or an alternative is used in the 5 most similar protocols

The fluorescence spectra of HTCC labeled with FITC (cHTCC-FITC = 0.5 μg/ml) in water were measured in the absence and in the presence of various concentrations of SARS-CoV-2 and MERS-CoV spike protein S1 domains ranging from 0.05 μg/ml to 0.4 μg/ml. Nuclear magnetic resonance (NMR) spectra were measured in deuterium oxide (D₂O) using a Bruker Advance II 600-MHz spectrometer. Elemental analysis was performed using a Vario Micro CHNS elemental analyzer. UV-Vis absorption spectra were recorded using a Varian Cary 50 UV-Vis spectrometer in 1-cm-path-length quartz cuvettes. Fluorescence spectra of HTCC-FITC were measured at an excitation wavelength (λ_ex) = 470 nm using a Hitachi F-7000 spectrofluorimeter at room temperature. Conductometric titrations were performed using an Elmetron CX-741 multifunction computer meter.

Milewska A., Chi Y., Szczepanski A., Barreto-Duran E., Dabrowska A., Botwina P., Obloza M., Liu K., Liu D., Guo X., Ge Y., Li J., Cui L., Ochman M., Urlik M., Rodziewicz-Motowidlo S., Zhu F., Szczubialka K., Nowakowska M, & Pyrc K. (2021). HTCC as a Polymeric Inhibitor of SARS-CoV-2 and MERS-CoV. Journal of Virology, 95(4), e01622-20.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!