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Mirna extraction kit and qpcr probes for specific mirnas

Manufactured by Qiagen
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The MiRNA extraction kit from Qiagen is designed to isolate and purify miRNA from a variety of sample types. The kit utilizes a spin column-based method to capture and extract miRNA molecules. The qPCR probes from Qiagen are specific for detecting and quantifying expression levels of select miRNA targets. These probes are compatible with real-time qPCR instrumentation and can be used to measure miRNA abundance in samples.

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Lab products found in correlation

Anti map2, by Abcam (2 mentions) Anti synj1, by Thermo Fisher Scientific (2 mentions) Anti ptau at8 and tau 5, by Thermo Fisher Scientific (2 mentions) Anti β actin and tubulin, by Santa Cruz Biotechnology (2 mentions) Anti holoapp mab348 and 6e10, by Merck Group (2 mentions) Anti beta amyloid, by Cell Signaling Technology (2 mentions) Texas red and alexa555 conjugated anti mouse and rabbit igg, by Thermo Fisher Scientific (2 mentions) Mir 195 inhibitors, by Applied Biological Materials (2 mentions) Am00100, by Applied Biological Materials (2 mentions) Amm1017200, by Applied Biological Materials (2 mentions) Amm3026700, by Applied Biological Materials (2 mentions) Anti rab5, by Santa Cruz Biotechnology (2 mentions)

Close spelling variants of this product

MiRNA extraction kit MiRNA probes QPCR kits

2 protocols using mirna extraction kit and qpcr probes for specific mirnas

1

Investigating Synj1 and Tau Proteins

Check if the same lab product or an alternative is used in the 5 most similar protocols
The anti-synj1 (rabbit polyclonal Ab, Novus, RRID: AB_11047653), anti-pTau AT8 and Tau-5 (ThermoFisher, RRID: AB_223647 and 10980631), anti-Rab5 (Santa Cruz Biotechnology, RRID:AB_628191), anti-β actin and tubulin (Santa Cruz Biotechnology, RRID:AB_476697 and 477498), anti-holoAPP MAB348 and 6E10 (Millipore RRID:AB_94882 and 564201), anti-beta-Amyloid (Cell Signaling Technology, RRID: AB_2056585), anti-MAP2 (Abcam, RRID:AB_297885), anti-dynamin clone 41 for (BD bioscience; RRID:AB_3976413), anti-mouse and rabbit HRP (ThermoFisher, RRID:AB_2556542 and 2540618), Texas-Red and Alexa555 conjugated anti-mouse and rabbit IgG (ThermoFisher, RRID:AB_10374713, 10983944, 2535987 and 1090271) were purchased. AAV2-containing miR-195, miR-374, scramble controls and miR-195 inhibitors were generated and obtained from ABM Inc. with detailed sequence information available (Am00100, Amm1017200 and Amm3026700). The miRNA extraction kit and qPCR probes for specific miRNAs were purchased from Exiqon Inc. The qPCR probes for actin (Hs1060665_g1), synj1 (Hs00953234_m1 and Mm01210539_m1), gapdh (Mm99999915_g1), RNU6B (NR_002752), 18 s and 45 s (4331182, Mm03928990_g1) were also purchased from ThermoFisher.
Cao J., Huang M., Guo L., Zhu L., Hou J., Zhang L., Pero A., Ng S., El Gaamouch F., Elder G., Sano M., Goate A., TCW J., Haroutunian V., Zhang B, & Cai D. (2020). MicroRNA-195 rescues ApoE4-induced cognitive deficits and lysosomal defects in Alzheimer’s disease pathogenesis. Molecular Psychiatry, 26(9), 4687-4701.
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2

Detailed Protocol for Analyzing Synaptic Proteins

Check if the same lab product or an alternative is used in the 5 most similar protocols
The anti-synj1 (rabbit polyclonal Ab, Novus, RRID: AB_11047653), anti-pTau AT8 and Tau-5 (ThermoFisher, RRID: AB_223647 and 10980631), anti-Rab5 (Santa Cruz Biotechnology, RRID:AB_628191), anti-β actin and tubulin (Santa Cruz Biotechnology, RRID:AB_476697 and 477498), anti-holoAPP MAB348 and 6E10 (Millipore RRID:AB_94882 and 564201), anti-beta-Amyloid (Cell Signaling Technology, RRID: AB_2056585), anti-MAP2 (Abcam, RRID:AB_297885), anti-dynamin clone 41 for (BD bioscience; RRID:AB_3976413), anti-mouse and rabbit HRP (ThermoFisher, RRID:AB_2556542 and 2540618), Texas-Red and Alexa555 conjugated anti-mouse and rabbit IgG (ThermoFisher, RRID:AB_10374713, 10983944, 2535987 and 1090271) were purchased. AAV2-containing miR-195, miR-374, scramble controls and miR-195 inhibitors were generated and obtained from ABM Inc. with detailed sequence information available (Am00100, Amm1017200 and Amm3026700). The miRNA extraction kit and qPCR probes for specific miRNAs were purchased from Exiqon Inc. The qPCR probes for actin (Hs1060665_g1), synj1 (Hs00953234_m1 and Mm01210539_m1), gapdh (Mm99999915_g1), RNU6B (NR_002752), 18s and 45s (4331182, Mm03928990_g1) were also purchased from ThermoFisher.
Cao J., Huang M., Guo L., Zhu L., Hou J., Zhang L., Pero A., Ng S., El Gaamouch F., Elder G., Sano M., Goate A., TCW J., Haroutunian V., Zhang B, & Cai D. (2020). MicroRNA-195 rescues ApoE4-induced cognitive deficits and lysosomal defects in Alzheimer’s disease pathogenesis. Molecular Psychiatry, 26(9), 4687-4701.
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